PMA - NF-κB Activator

Phorbol myristate acetate

ABOUT

Phorbol myristate acetate. NF-κB Activator

Phorbol 12-myristate 13-acetate (PMA), also known as 12-O-tetradecanoylphorbol 13-acetate (TPA), is a specific activator of Protein Kinase C (PKC) and hence activates nuclear factor-kappa B (NF-κB). NF-κB is a transcription factor that regulates numerous physiological functions and is involved in the pathogenesis of various diseases. It has been identified as a potential therapeutic target in inflammatory processes, cancer, and autoimmune diseases[1].

PMA is the most common and potent phorbol ester. It is active at nanomolar concentrations and activates NF-κB in a dose-dependent manner [1]. PMA causes a wide range of effects in cells and tissues and is a very potent mouse skin tumor promoter [2, 3].

InvivoGen’s PMA is designed to study the NF-κB pathway in cellular assays.

 

Key Features of PMA:

  • Potent activator of NF-κB in vitro
  • Each lot of PMA is highly pure (≥98%) and functionally tested

 

References:

1. Hellweg C.E. et al., 2006. Activation of nuclear factor kappa B by different agents: influence of culture conditions in a cell-based assay. Ann N Y Acad Sci. 1091:191-204.
2. Chang MS. et al., 2005. Phorbol 12-myristate 13-acetate upregulates cyclooxygenase-2 expression in human pulmonary epithelial cells via Ras, Raf-1, ERK, and NF-kappaB, but not p38 MAPK, pathways. Cell Signal. 17(3):299-310.
3. Fürstenberger G. et al., 1981. Skin tumor promotion by phorbol esters is a two-stage process. PNAS. 78(12):7722-6.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Synonyms
12-O-tetradecanoylphorbol 13-acetate (TPA)
Phorbol 12-myristate 13-acetate
Tested applications

In vitro cellular assays

CAS number
16561-29-8
Chemical formula

C36H56O8

Molecular weight
616.8 g/mol
Purity
≥ 98% (UHPLC)
Solubility

5 mg/ml in DMSO

Working concentration

10 ng - 1 µg/ml

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    PMA
  • Cat code: 
    tlrl-pma
  • Quantity: 
    5 mg
Includes:

1.5 ml of sterile endotoxin-free water

Notes:

PMA is provided as a translucent film

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: The reconstituted product is stable up to 6 months at -20 °C.

    Caution:

    • Avoid repeated freeze-thaw cycles
    • Protect from light

DOCUMENTS

Documents

PMA

Technical Data Sheet

Validation Data Sheet

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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