pFUSE-hIgG1e2-Fc
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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pFUSE-hIgG1e2-Fc1
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Show product |
20 µg |
pfc1-hg1e2
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pFUSE-hIgG1e2-Fc2
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Show product |
20 µg |
pfc2-hg1e2
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pFUSE-hIgG1e2-Fc1 is a cloning plasmid for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the mutated human IgG1 heavy chain and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each part of the molecule to function independently. The engineered hIgG1e2 Fc contains the following mutations: M252Y/S254T/T256E and H433K/N434F.
pFUSE-hIgG1e2-Fc2 additionally features the IL2 signal sequence for the generation of Fc-Fusion proteins derived from proteins that are not naturally secreted.
Vaccaro C. et al., 2005. Engineering the Fc region of immunoglobulin G to modulate in vivo antibody levels. Nat Biotechnol. 23(10):1283-8.
Back to the topSpecifications
Isotype: IgG1 human
Mutations: M252Y/S254T/T256E + H433K/N434F
Characteristics:
- Increased binding to neonatal FcR (FcRn)
- Increased half-life
Both plasmids are selectable with Zeocin™.
Back to the toppFUSE Contents
- 20 µg of lyophilized DNA
- 1 ml of Zeocin® (100 mg/ml)
Product is shipped at room temperature