pFUSE-CHIg-mG2a
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Cat.code:
pfuse-mchg2a
- Documents
ABOUT
Murine IgG2a heavy chain constant region
pFUSE-CHIg-mG2a is a cloning plasmid that expresses the constant region of the mouse IgG2a heavy chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain (CH) variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.
pFUSEss-CHIg-mG2a additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv) fragments selected from phage display libraries that lack a signal sequence.
Key features of pFUSE-CHIg-mG2a
- Encodes the murine IgG2a CH constant region
- Long-lasting expression due to the composite hEF1-HTLV promoter
- Selectable with Zeocin® in both bacterial and mammalian cells
- Available with or without the IL-2 signal sequence
All products are for internal research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
Antibody generation and isotype switching
Plasmid construct is confirmed by restriction analysis and full-length open reading frame (ORF) sequencing.
CONTENTS
Contents
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Product:pFUSE-CHIg-mG2a
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Cat code:pfuse-mchg2a
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Quantity:20 µg
1 ml of Zeocin® (100 mg/ml)
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
DOCUMENTS
Documents
Technical Data Sheet
Plasmid Sequence
Safety Data Sheet
Certificate of analysis
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