pDRIVE-Tyr

Tyr promoter (Melanocytes & melanoma) in pDRIVE expression plasmid

ABOUT

Tyrosinase promoter in pDRIVE expression plasmid

Tyrosinase, the rate-limiting enzyme in melanin synthesis, is expressed specifically in pigment-producing cells. The expression of tyrosinase is regulated by cyclic AMP (cAMP).

The M-box 70-bp upstream from the TATA-box and the E-box located downstream of the TATA-box, near the initiator site, are involved in the regulation of the tyrosinase promoter activity by cAMP [1].

Melanoma-specific expression of a transgene can be achieved by using the tyrosinase promoter [2], this targeted expression being further amplified by the addition of the melanocyte-specific enhancer.

 

References:

1. Bertolotto C. et al. 1996. Regulation of tyrosinase gene expression by cAMP in B16 melanoma cells involves two CATGTG motifs surrounding the TATA box: implication of the microphthalmia gene product. J Cell Biol. 134(3):747-55.
2. Vile R. et al. 1994. A comparison of the properties of different retroviral vectors containing the murine tyrosinase promoter to achieve transcriptionally targeted expression of the HSVtk or IL-2 genes. Gene Ther. 1(5):307-16.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Plasmid backbone
pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia
Antibiotic resistance
Zeocin®
Gene promoter
Tyrosinase
Specificity

Melanocytes & melanoma

Reporter gene
Lac Z
Purification
Ion-exchange chromatography
Appearance (form)
Translucent film
Appearance (color)
Colorless
Reconstitution buffer
Sterile water (not provided)
Tested applications

In vitro transfection

Quality control

Plasmid construct is confirmed by restriction analysis and full-length open reading frame (ORF) sequencing.

CONTENTS

Contents

  • Product: 
    pDRIVE-mTyr
  • Cat code: 
    pdrive-mtyr
  • Quantity: 
    20 µg
Includes:

1 ml of Zeocin™ (100 mg/ml)

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • Upon receipt, store product at -20 °C
    Stability: Reconstituted product is stable for at least 1 year at -20 °C.

DOCUMENTS

Documents

pDRIVE-mTyr

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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