pDRIVE-ROSA

ROSA promoter (Ubiquitous) in pDRIVE expression plasmid

The ROSA26 promoter, initially identified by random retroviral gene trapping in mouse embryonic stem cells [1], directs expression of reporter [2] and recombinase genes [3] in all cells throughout embryonic development and in adult tissues.

This TATA-less promoter is very effective in vitro in a very broad range of mammalian cell lines.

The strength of the ROSA26 promoter is ascribed to the 10 potential Sp1 sites found within the CpG island extending from the proximal promoter to the first half of intron 1, the highest number of Sp1 sites ever recorded in any natural promoter.

References:

1. Zambrowicz BP. et al. 1997. Disruption of overlapping transcripts in the ROSA beta geo 26 gene trap strain leads to widespread expression of beta-galactosidase in mouse embryos and hematopoietic cells. Proc Natl Acad Sci USA. 94:3789-94.
2. Kisseberth WC. et al. 1999. Ubiquitous expression of marker transgenes in mice and rats. Dev Biol.214:128-38.
3. Farley FW. et al. 2000. Widespread recombinase expression using FLPeR (Flipper) mice. Genesis. 28:106-10.

Specifications

pDRIVE bearing human ROSA promoter - LacZ, SEAP or Lucia

Gene Promoter: ROSA

Specificity: Ubiquitous

Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia

Selection: Zeocin™

pDRIVE bearing mouse ROSA promoter - LacZ, SEAP or Lucia

Gene: ROSA

Specificity: Ubiquitous

Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia

Selection: Zeocin™

pDRIVE contents

• 20 µg of lyophilized DNA
• 1 ml of Zeocin™ (100 mg/ml)

Product is shipped at room temperature

Details