pDRIVE-PSA / PSA
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
|---|---|---|---|---|---|---|
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pDRIVE-PSA-hPSA pDRIVE bearing human PSA / PSA promoter - LacZ |
Show product |
20 µg |
pdrive-psahpsa
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pDRIVE5SEAP-PSA-hPSA pDRIVE bearing human PSA / PSA promoter - SEAP |
Show product |
20 µg |
pdrive5s-psahpsa
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pDRIVE5Lucia-PSA-hPSA pDRIVE bearing human PSA / PSA promoter - Lucia |
Show product |
20 µg |
pdrive5lc-psahpsa
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Prostate-specific antigen (PSA) enhancer and promoter in pDRIVE expression plasmid
Prostate-specific antigen (PSA) is a serine protease primarily synthesized by both normal prostate epithelium and the vast majority of prostate cancers.
PSA expression is mainly induced by androgens at the transcriptional level via the androgen receptor (AR). The AR modulates transcription through its interaction with its consensus DNA binding site, GGTACAnnnTGTT/CCT, termed the androgen response element (ARE) [1].
The core PSA promoter region exhibits low activity and specificity, but the inclusion of the PSA enhancer sequence which contains a putative ARE increases expression, specifically in PSA-positive cells. The expression can be further increased when induced with androgens such as dihydrotestosterone [2].
References:
1. Schuur ER. et al. 1996. Prostate-specific antigen expression is regulated by an upstream enhancer. JBC. 271(12):7043-7051.
2. Latham JP. et al. 2000. Prostate-specific antigen promoter/enhancer driven gene therapy for prostate cancer: construction and testing of a tissue-specific adenovirus vector. Cancer Res. 60(2):334-41.
Specifications
pDRIVE bearing human PSA / PSA promoter - LacZ, SEAP or Lucia
Gene Promoter: PSA : Prostate Specific Antigen
Specificity: Prostate and prostate cancers
Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia
Selection: Zeocin™
pDRIVE contents
- 20 µg of lyophilized DNA
- 1 ml of Zeocin™ (100 mg/ml)
Product is shipped at room temperature
