pDRIVE-Mb

Mb promoter (Muscle) in pDRIVE expression plasmid

Myoglobin is a cytoplasmic hemoprotein that binds molecular oxygen to facilitate its diffusion from the vascular system to the muscle mitochondria.

Myoglobin is found only in muscle, predominantly in cardiac and skeletal myocytes.

he myoglobin promoter can drive the expression of a transgene in multinucleated myotubes but not in  mononucleated and undifferentiated cells [1].

This capacity is conferred by a small segment of the 5’ flanking region extending from nucleotides -357 to +55 relative to the transcriptional start site [2].

References:

1. Devlin BH. et al. 1989. Identification of a muscle-specific enhancer within the 5’-flanking region of the human myoglobin gene. J Biol Chem 264(23):13896-13901.
2. Yan Z. et al. 2001. Regulatory elements governing transcription in specialized myofiber subtypes. J Biol Chem 276(20):17361-17366.