pCpGfree-promoter (mSEAP)
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Cat.code:
pcpgf-prom
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ABOUT
Expression vector CpG-free for enhancer CpG methylation study
pCpGfree-promoter is a reporter plasmid completely devoid of CpG dinucleotides.
This plasmid contains a CpG-free allele of a reporter gene (murine SEAP or secreted luciferase Lucia), the human EF-1α promoter and a multiple cloning site in place of the enhancer.
It is specifically designed to analyze the effect of methylation on CpG residues present in enhancer elements.
Disclaimer: This product is covered by a Limited Use License (See Terms and Conditions).
All InvivoGen products are for internal research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
in vitro transfection
Plasmid construct has been confirmed by restriction analysis and sequencing.
CONTENTS
Contents
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Product:pCpGfree-promoter (mSEAP)
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Cat code:pcpgf-prom
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Quantity:20 µg
E. coli GT115 strain provided lyophilized on a paper disk, 1 ml of Zeocin™ (100 mg/ml)
Shipping & Storage
- Shipping method: Room temperature
- -20°C
Storage:
Details
MCS: 5' - SdaI, Bsp120I, AvrII, NsiI, Ppu10I, ScaI, BamHI, SpeI - 3'
Procedure
1- Insert promoter and/or enhancer fragment into pCpGfree-promoter.
2- Treat recombinant plasmid with CpG methylase (Sss I) or site specific-methylase (Hha I, Hpa II).
3- Transiently transfect cells with methylase-treated or untreated plasmid.
4- Analyze promoter CpG methylation by determining reporter expression using the appropriate detection reagent.
DOCUMENTS
Documents
Technical Data Sheet
Plasmid Sequence
Safety Data Sheet
Certificate of analysis
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