G3-YSD - cGAS Agonist

Y-form DNA

ABOUT

cGAS Agonist

G3-YSD (G3-ended Y-form Short DNA) is a 26-mer DNA sequence derived from the HIV-1 RNA genome [1]. This short DNA displays a Y-shape arising from its palindromic sequence flanked by unpaired guanosine trimers (G3). The guanosine overhangs in this Y-form DNA have been identified as minimal recognition motifs for cGAS (cyclic GMP-AMP synthase, cGAMP synthase), a critical cytosolic DNA sensor [1].

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Mode of action:

cGAS detects double-stranded DNA (dsDNA) over 40 bp in length, or stem-loop structures of single-stranded DNA (ssDNA) flanked by unpaired nucleotides [1-3]. Interaction of cGAS with its agonists promotes the synthesis of 2’3’-cGAMP, a second messenger that activates STING (stimulator of interferon genes), and the downstream production of type I interferons (IFNs) and other cytokines [2]. Response to G3-YSD is strictly cGAS-dependent, as demonstrated by the lack of type I IFN production upon intracellular delivery of G3-YSD in cGAS-KO cells (see figures).

 

Key features of G3-YSD:

  • Potent cGAS-specific agonist
  • Y-form Short DNA flanked with guanosine trimers
  • Each lot is functionally tested

 

Read our review on cGAS.

 

References:

1. Herzner A.M. et al., 2015. Sequence-specific activation of the DNA sensor cGAS by Y-form DNA structures as found in primary HIV-1 cDNA. Nat Immunol. 16(10):1025-33.
2. Li T. & Chen Z.J., 2018. The cGAS-cGAMP-STING pathway connects DNA damage to inflammation, senescence, and cancer. J Exp Med.215(5):1287-1299.
3. Luecke S. et al., 2017. cGAS is activated by DNA in a length-dependent manner. EMBO Rep. 18(10):1707-1715.

All products are for research use only, and not for human or veterinary use.

cGAS activation with G3-YSD
cGAS activation with G3-YSD

SPECIFICATIONS

Specifications

Sequence

5’ GGG TATATATATGCATATATATA GGG 3’ (26 mer)

Molecular weight
8088.1 g/mol
Working concentration

100 ng - 1 μg/ml

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    G3-YSD
  • Cat code: 
    tlrl-ydna
  • Quantity: 
    200 µg
Includes:

1.5 ml sterile endotoxin-free water

Notes:

G3-YSD contains a palindromic sequence for which self-hybridization results in double-stranded DNA. The flanking guanosine trimers in 5' and 3' remain unpaired.

Shipping & Storage

  • Shipping method:  Room temperature

    • Storage:

    • -20°C

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

cGAS

Cyclic GMP-AMP synthase (cGAS, cGAMP synthase) is a critical cytosolic DNA sensor that triggers innate immune responses through the production of type I interferons (IFNs) [1]. In response to cytosolic double‑stranded DNA (dsDNA), cGAS produces the cyclic dinucleotide (CDN) 2’3’-cGAMP.
CDNs bind directly to STING, leading to TBK1‑IRF3-mediated activation of IFN-stimulated response elements (ISRE) in the promoters of IFN-stimulated genes (ISG). The most potent agonist of human STING is 2’3’-cGAMP [2,3].

 

References:

1. Sun L. et al., 2013. Cyclic GMP-AMP synthase is a cytosolic DNA sensor that activates the type I interferon pathway. Science 339(6121):786-91.
2. Gao P. et al., 2013. Cyclic [G(2’,5’)pA(3’,5’)p] is the metazoan second messenger produced by DNA-activated cyclic GMP-AMP synthase. Cell. 153(5):1094-107.
3. Ablasser A. et al., 2013. cGAS produces a 2’-5’-linked cyclic dinucleotide second messenger that activates STING. Nature. 498(7454):380-4.

DOCUMENTS

Documents

G3-YSD

Technical Data Sheet

Validation Data Sheet

Safety Data Sheet

Certificate of analysis

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