Human IL-6R (CD126) Antibody - Tocilizumab IgGA2 Isotype

Human IgA2

ABOUT

Anti-human IL-6R antibody - Human IgA2 (low effector functions)

Anti-hIL-6R-hIgA2 is an Tocilizumab biosimilar with an IgA2 isotype. Tocilizumab, also known as TCZ, is a recombinant, humanized monoclonal antibody (mAb) directed against both soluble and membrane-bound human IL-6R. Tocilizumab is FDA-approved for the treatment of inflammatory diseases and conditions such as rheumatoid arthritis (RA) and cytokine release syndrome.

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Anti-hIL-6R-hIgA2 comprises the variable region of Tocilizumab and an IgA2 constant region mediating low effector functions.

This antibody can be used with HEK-Blue™ IL-6 cells for screening and neutralization assays to block recombinant human IL-6-induced signaling (see figure).

Key features

  • Each lot is functionally tested and validated
  • The complete sequence of the antibody construct has been verified
  • Absence of endotoxins determined by the EndotoxDetect™ assay
     
     

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Target

IL-6R, CD126

Target species

Human

Species
Human
Isotype
hIgA2
kappa
Clone
Tocilizumab
Synonyms
Actemra
Anti-IL-6 mAb
CAS#375823-41-9
Tag
Tag-free
Source
CHO cells
Production details
Animal-free
Purification
Protein M
Formulation buffer

Sodium phosphate buffer, glycine, saccharose, stabilizing agents

Preservative
Azide-free
Purity
≥ 95 %
Appearance (form)
Lyophilized
Reconstitution buffer
Sterile water (not provided)
Endotoxin

Negative (tested using EndotoxDetect™ assay)

Tested applications

Cellular assay, ELISA, flow cytometry, Fc interaction studies

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    Anti-hIL6R-To-hIgA2
  • Cat code: 
    hil6rto-mab7
  • Quantity: 
    100 µg

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: -20°C for up to 1 year

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

IL-6R background

Tocilizumab (TCZ) is a recombinant, humanized monoclonal antibody (mAb) directed against both soluble and membrane-bound human IL-6R. TCZ exhibits anti-inflammatory activity by inhibiting the binding of the pro-inflammatory cytokine, IL-6 to its receptor [1]. IL-6 exerts its biological effects through IL-6R, and the transmembrane protein gp130. Despite only a few cells expressing IL-6R on their surface, many cells respond to IL-6 due to the existence of soluble IL-6R [2]. TCZ can block both modes of signaling [1]. TCZ has been approved for the treatment of inflammatory diseases and conditions such as rheumatoid arthritis (RA) and cytokine release syndrome (CRS), a side effect of CAR-T therapy. Furthermore, it is under investigation for the treatment of the late-stage hyperinflammation observed in severe COVID‑19 patients [3].

 

Learn more on SARS-CoV-2 Read our reviews on COVID-19 and learn more about SARS-CoV-2 infection cycle, immune responses, and potential therapeutics.

 

References

1. Sheppard, M. et al. 2017. Tocilizumab (Actemra). Hum Vaccin Immunother 13, 1972- 1988.
2. Rose-John, S. 2012. IL-6 trans-signaling via the soluble IL-6 receptor: importance for the pro-inflammatory activities of IL-6. Int J Biol Sci 8, 1237-1247.
3. Zhang, S. et al. 2020. Rational Use of Tocilizumab in the Treatment of Novel Coronavirus Pneumonia. Clin Drug Investig.

DOCUMENTS

Documents

Anti-hIL6R-To-hIgA2

Technical Data Sheet

Safety Data Sheet

Validation Data Sheet

Certificate of analysis

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