Anti-human Dectin-2 (CLEC6A) antibody

Human Dectin-2 neutralizing and detection antibody - Monoclonal mouse IgG2a

Neutralizing and detection antibody against hDectin-2

Anti-hDectin-2-IgG (clone Q7-4B5) is a monoclonal mouse IgG2a antibody against human Dectin-2 (hDectin-2). This antibody was screened for neutralization activity and flow cytometry. It is produced in hybridomas and purified by affinity chromatography with protein G.  

Dendritic cell-associated C-type lectin-2 (Dectin-2) is a C-type lectin receptor (CLR), encoded by the C-type lectin domain containing 6A (CLEC6A) gene. It plays an important role in antifungal innate immunity [1, 2]. It binds high mannose-type carbohydrates and has been demonstrated to be the functional receptor for α-mannans.

More details

Key features:

• Targets human Dectin-2
• Provided azide-free
• Each lot is functionally tested

Read our review on C-Type Lectin Receptors

References:

1. Hou H. et al., 2017. C-type Lectin Receptor: Old Friend and New Player. Med Chem. 2017;13(6):536-543.
2. Wang H.L. et al., 2017. Ligation of Dectin-2 with a novel microbial ligand promotes adjuvant activity for vaccination. PLoS Pathog. 213(8):e1006568.

Specifications

Target: Human Dectin-2 (hDectin-2)

Specificity: No cross-reactivity with murine Dectin-2

Clonality: Monoclonal antibody

Clone: Q7-4B5

Isotype: Mouse IgG2a, kappa

Control: Mouse Control IgG2a

Source: Hybridoma cells

Formulation: 0.2 µm filtered solution in a sodium phosphate buffer with saccharose, glycine, and stabilizing agents.

Purity: Purified by affinity chromatography with protein G

Applications: Neutralization/block; Flow cytometry

Quality control:

• This product has been validated for neutralization using cellular assays.
• Binding of Anti-hDectin-2-IgG to hDectin-2 on cells has been validated using flow cytometry.
• The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.

Contents

• 2 x 100 µg of purified Anti-hDectin-2-IgG, provided azide-free and lyophilized

Product is shipped at room temperature.

 Upon receipt, store lyophilized antibody at -20°C.

Details

Dectin-2 is a member of the C-type lectin receptor (CLR) family and plays an important role in antifungal innate immunity [1].
CLRs comprise a large family of receptors that bind to carbohydrates in a calcium-dependent manner. The lectin activity of these receptors is mediated by conserved carbohydrate-recognition domains (CRDs).
Dectin-2 is a type II transmembrane CLRs with a single CRD domain. Dectin-2 binds high mannose-type carbohydrates and was shown to be the functional receptor for a-mannans. Upon binding to its ligand, Dectin-2 signals through the kinase Syk and the adaptors CARD9/Bcl-10/MALT1 triggering the activation of NF-kB and the subsequent production of pro-inflammatory cytokines [1, 2].
Dectin-2 is implicated in anti-bacterial immunity and allergy [3]. In fact, it has been demonstrated that Dectin-2 is important for the induction of Th17 in response to fungal infection [4].

References

1. Sancho D & Reis e Sousa C., 2012. Signaling by myeloid C-type lectin receptors in immunity and homeostasis. Annu Rev Immunol. 30:491-529.
2. Hernández-Santos N. & Gaffen SL., 2012. Th17 cells in immunity to Candida albicans. Cell Host Microbe. 11(5):425-35.
3. Drummond R. et al., 2011. The role of Syk/CARD9 coupled C-type lectins in antifungal immunity. Eur J Immunol. 41:276-81.
4. Saijo S. et al., 2010. Dectin-2 recognition of alpha-mannans and induction of Th17 cell differentiation is essential for host defense against Candida albicans. Immunity. 32(5):681-91.