Anti-HLA Class II Control

Monoclonal IgG1 positive control for class II Luminex® (LABScreen® or LIFECODES HLA)

IgG1 is the most abundant IgG in serum (66%). IgG1 antibodies strongly activate complement and exhibit strong cellular effector function.

Anti-HLA Class II Control is a chimeric human IgG1 monoclonal antibody specifically designed for studying detection of human IgG antibodies to HLA class II. Its constant regions are from human IgG1 (i.e. human IgG1 heavy chains and kappa light chains) and its variable regions are derived from the murine monoclonal anti-HLA class II antibody F3.3 [1].

Anti-HLA Class II Control can be used as an IgG positive control for IgG anti-HLA antibody detection assays, including LABScreen®, LIFECODES HLA and C1qScreen™.

Anti-HLA Class II Control has been validated in HLA Class II LABScreen® Mixed and LABScreen® Single-Antigen. It reacts with all HLA-DP, DR and DQ2 antigens but not with DQ1, DQ3 & DQ4 [2].

The anti-HLA Class II Control also binds strongly to recombinant C1q, using the C1qScreen™ (see figures).

References:

1. Elsässer D. et al., 1996. HLA class II as potential target antigen on malignant B cells for therapy with bispecific antibodies in combination with granulocyte colony-stimulating factor. Blood. 87(9):3803-12.
2. Congy-Jolivet N et al. 2013. Production and characterization of chimeric anti-HLA monoclonal antibodies targeting public epitopes as tools for standardizations of the anti-HLA antibody detection. J Immunol Methods. 390(1-2):41-51