Anti-HLA Class I Control

Monoclonal IgG1 positive control for class I Luminex® (LABScreen® or LIFECODES HLA)

IgG1 are the most abundant IgG present in serum (66%). They have a high complement-activating capacity and important cellular effector function.

Anti-HLA Class I Control is a chimeric human IgG1 monoclonal antibody designed for studying detection of human IgG antibodies to HLA class I. Its constant regions are from human IgG1 (i.e. human IgG1 heavy chains and kappa light chains) and its variable regions are derived from the murine monoclonal anti-HLA class I W6/32 [1, 2].

Anti-HLA Class I Control can be used as an IgG positive control for IgG anti-HLA antibody detection assays, including LABScreen®, LIFECODES HLA and C1qScreen™.

Anti-HLA Class I Control has been validated on LABScreen® Mixed and LABScreen® Single Antigen HLA Class I. It reacts with all class I HLA-A, B and Cw antigens [3]. The anti-HLA Class I IgG1 Control binds strongly to recombinant C1q using the C1qScreen™ kit (see figures).

References:

1. Barnstable CJ. et al., 1978. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis. Cell. 14(1):9-20.
2. Hilton HG, Parham P., 2013. Direct binding to antigen-coated beads refines the specificity and cross-reactivity of four monoclonal antibodies that recognize polymorphic epitopes of HLA class I molecules. Tissue Antigens. 81(4):212-20
3. Congy-Jolivet N et al. 2013. Production and characterization of chimeric anti-HLA monoclonal antibodies targeting public epitopes as tools for standardizations of the anti-HLA antibody detection. J Immunol Methods. 390(1-2):41-51

Figures

Typical fluorescent dose-response obtained by serial dilution of Anti-HLA Class I control (hIgG1) antibody (μg/ml) on LABScreen® Mixed labeled with a PE-conjugated anti-human IgG.

The curve is a plot of average MFI values for the class I beads (minimal standard deviation as each bead gave a very similar value). The class II beads signal (not shown) was at background level.

Fluorescent (MFI) dose response obtained by binding of anti-HLA class I control to LABScreen® Single Antigen beads.

Each curve is representative of a specific HLA antigenic bead. Antigens that gave the lowest values are indicated on the rights As observed in the graph, all class I antigens are recognized by the anti-HLA class I control.

Average fluorescent intensity obtained at 1 µg/ml of Anti-HLA Class I Control (hIgG1) on LABScreen® Mixed and C1qScreen™. The data are average MFIs of the class I beads. Human IgG2 (hIgG2), hIgG3 and hIgG4 anti-HLA antibodies were produced separately to assess sensitivity. The class II beads signal (not shown) was at background level.

Specifications

Working concentration: 0.2 ng/ml – 2 µg/ml

Specificity: class I HLA-A, B and Cw antigens.

Isotype: Human IgG1, kappa

Formulation: 0.2 µm filtered solution in PBS with 5% saccharose

Applications: positive controls for all anti-HLA antibody detection assays, including:

• multiplex bead array assays using Luminex® technology (LABScreen®, LIFECODES HLA, C1qScreen™);
• ELISA;
• indirect immunofluorescence techniques (flow cytometry);
• complement-dependent lymphocytotoxicity.

Contents

• 100 μg sterile Anti-HLA Class I Ctrl antibody, provided azide-free and lyophilized

Store the antibody at -20°C. 

Lyophilized product is stable 24 months at -20°C.