Anti-hCD14-hIgG1

Neutralizing and detection IgG monoclonal antibody to human CD14

Neutralizing monoclonal antibody against human CD-14

Anti-hCD14-hIgG1 (clone D3B8) is a chimeric monoclonal antibody specific for human CD14. This antibody was generated by recombinant DNA technology. It has been produced in Chinese hamster ovary (CHO) cells and purified by affinity chromatography.  Anti-hCD14-hIgG1 has been selected for its ability to efficiently neutralize the biological activity of hCD14. It can also be used to detect hCD14 using flow cytometry.
CD14 is a glycosylphosphatidylinositol (GPI)-anchored membrane protein known to serve as a co-receptor for several TLRs [1]. Notably, CD14 serves as a member of the heteromeric lipopolysaccharide (LPS) receptor complex that also contains TLR4 and MD2 [2].  Furthermore, CD14 was shown to interact with TLR2 in response to various microbial infections [3].

More details

Key features:

• Reacts with human CD14
• Provided azide-free
• Each lot is functionally tested

Read our review on Toll-Like Receptors.

References:

1. Pugin J. et al., 1994. CD14 is a pattern recognition receptor. Immunity. 1(6):509-16.
2. Da Silva Correia J. et al., 2001. Lipopolysaccharide is in close proximity to each of the proteins in its membrane receptor complex. transfer from CD14 to TLR4 and MD-2. J Biol Chem. 276(24): 21129-35.
3. Aderem A. & Ulevitch R.J., 2000. Toll-like receptors in the induction of the innate immune response. Nature. 406(6797):782-7.

Specifications

Clonality: Monoclonal antibody

Specificity: Human CD14 (hCD14)

Clone: D3B8

Source: CHO cells

Isotype: Human IgG1, kappa

Control: Human IgG1 control

Formulation: 0.2 μm filtered solution in sodium phosphate buffer with glycine, saccharose, and stabilizing agents

Applications: Neutralization/block; Flow cytometry

Quality control:

• The biological activity has been validated using cellular assays.
• The binding of Anti-hCD14-IgG to hCD14 has been validated using flow cytometry.
• The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.

Contents

• 2 x 100 µg of purified Anti-hCD14-IgG provided azide-free and lyophilized.

Product is shipped at room temperature.

Upon receipt, store lyophilized antibody at -20 °C.

Details

CD14 is a glycosylphosphatidylinositol (GPI)-anchored membrane protein that acts as a bacterial pattern recognition receptor [1]. CD14 is found on cells derived from the monocyte/macrophage lineage, as well as neutrophils and B lymphocytes. CD14 serves as a member of the heteromeric lipopolysaccharide (LPS) receptor complex that also contains TLR4 and MD2 [2]. CD14 binds LPS but is not capable of initiating a transmembrane activation signal since it does not contain a cytoplasmic domain. Upon LPS binding, CD14 physically associates with TLR4 which in turn transduces the signal. CD14 was also shown to interact with TLR2 in response to various microbial infections [3].

1. Pugin J. et al., 1994. CD14 is a pattern recognition receptor. Immunity. 1(6):509-16.
2. Da Silva Correia J. et al., 2001. Lipopolysaccharide is in close proximity to each of the proteins in its membrane receptor complex. transfer from CD14 to TLR4 and MD-2. J Biol Chem. 276(24): 21129-35.
3. Aderem A. & Ulevitch R.J., 2000. Toll-like receptors in the induction of the innate immune response. Nature. 406(6797):782-7.