pDRIVE-OG-2

OG-2 promoter (Osteoblasts, Odonblasts) in pDRIVE expression plasmid

Osteocalcin gene 2 (OG2) expression is restricted to mature osteoblasts and odontoblasts and is regulated at the transcriptional level by hormones like Vit D3 and growth factors like TNFα.

The OG2 proximal promoter located between -147 and -34 is sufficient to confer osteoblast-specific expression to a reporter gene [1].

This region contains two osteoblast-specific cis-acting elements called OSE1 and OSE2 [2]. Multimers of each of these elements confer osteoblast specific activity to a heterologous promoter.

Addition of other regulatory elements located between 647 and 147 can further increase the expression level of a transgene in vivo [3].

References:

1. Ducy P. & Karsenty G. 1995. Two distinct osteoblast-specific cis-acting elements control expression of a mouse osteocalcin gene. Mol Cell Biol 15(4):1858-69.
2. Schinke T. & Karsenty G. 1999. Characterization of Osf1, an osteoblast-specific transcription factor binding to a critical cis-acting element in the mouse Osteocalcin promoters. J Biol Chem. 274(42):30182-9.
3. Frendo JL. et al. 1998. Functional hierarchy between two OSE2 elements in the control of osteocalcin gene expression in vivo. J Biol Chem 273(46):30509-16.