pDRIVE-Elastase-1

Elastase-1 promoter (Pancreatic acinar cells) in pDRIVE expression plasmid

The elastase gene is one of the pancreatic digestive enzyme genes which is activated in a stage- and site- specific manner during development of the mammalian gut.

The rat elastase 1 promoter is a 213 bp fragment comprised of the core promoter (-72 to +8) and the enhancer (-205 to -73).

The elastase 1 promoter was shown to direct the expression of a human growth hormone transgene in pancreatic acinar cells, stomach, duodenum, and colon.

This pattern of selective expression limited to the gastroenteropancreatic organ system is specified by the A element, one of three functional elements in the elastase 1 enhancer.

References:

1. Ornitz DM. et al. 1985. Specific expression of an elastase-human growth hormone fusion gene in pancreatic acinar cells of transgenic mice. Nature 313(6003):600-2.
2. Rose SD. et al. 1994. A single element of the elastase I enhancer is sufficient to direct transcription selectively to the pancreas and gut. Mol Cell Biol 14(3):2048-57.