pDRIVE-Flt-1
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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pDRIVE-FLT1 pDRIVE bearing human Flt-1 promoter - LacZ |
Show product |
20 µg |
pdrive-hflt1
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pDRIVE5SEAP-FLT1 pDRIVE bearing human Flt-1 promoter - SEAP |
Show product |
20 µg |
pdrive5s-hflt1
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pDRIVE5Lucia-FLT1 pDRIVE bearing human Flt-1 promoter - Lucia |
Show product |
20 µg |
pdrive5lc-hflt1
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Flt-1 promoter (Endothelial cells) in pDRIVE expression plasmid
The fms-like tyrosine kinase Flt-1 is one of the receptors for VEGF.
Flt-1 is expressed specifically in endothelial cells and up-regulated in tumor vasculature. The promoter region contains a TATA box, a GC-rich region, nine Ets motifs and one CRE motif, located in a 489 bp fragment upstream of exon-1 [1].
This region is essential for basal and cell-type specific expression of the Flt-1 gene [2].
The Flt-1 promoter has been used to generate high levels of transgene expression targeted to endothelial cells in vitro and in vivo [3].
References:
1. Morishita K. et al., 1995. A novel promoter for vascular endothelial growth factor receptor (flt-1) that confers endothelial-specific gene expression. J Biol Chem. 270(46):27948-53.
2. Wakiya K. et al., 1996. AcAMP response element and an Ets motif are involved in the transcriptional regulation of flt-1 tyrosine kinase (vascular endothelial growth factor receptor 1) gene. J Biol Chem. 1996 Nov 29;271(48):30823-8.
3. Nicklin SA. et al., 2001. Analysis of cell-specific promoters for viral gene therapy targeted at the vascular endothelium.
Hypertension. 38(1):65-70.
Specifications
pDRIVE bearing human Flt-1 promoter - LacZ, SEAP or Lucia
Gene Promoter: VEGF Receptor 1
Specificity: Endothelial cells
Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia
Selection: Zeocin™
Back to the toppDRIVE contents
- 20 µg of lyophilized DNA
- 1 ml of Zeocin™ (100 mg/ml)
Product is shipped at room temperature
