pDRIVE-mIFN-β

Mouse IFN-β promoter (Hematopoietic cells) in pDRIVE expression plasmid

Expression of the interferon-beta (IFN-β) gene is induced by a variety of agents, including viruses and double-stranded RNA (dsRNA), and is also strongly repressed in uninduced cells, a requirement imposed by the cytotoxic properties of IFN-β.

The functional IFN-β promoter resides within a 170 bp fragment located 5’ of the coding sequence [1].

This region contains four binding sites for transcription factors such as NF-κB and IRF-1 that act as activators, as well as putative negative regulatory domains.

Fusion of the IFN-β promoter region to the luciferase gene was shown to render the expression of the reporter gene inducible by dsRNA [2].

References:

1. Dirks W. et al., 1989. Isolation and functional characterization of the murine interferon-beta 1 promoter. J Interferon Res. 9(1):125-33.
2. King P& Goodbourn S. 1994. The betainterferon promoter responds to priming through multiple independent regulatory elements. J Biol Chem. 269(48):30609-15.