pDRIVE-NphsI
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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pDRIVE-hNphsI pDRIVE bearing human NphsI promoter - LacZ |
Show product |
20 µg |
pdrive-hnphs
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pDRIVE5SEAP-hNphsI pDRIVE bearing human NphsI promoter - SEAP |
Show product |
20 µg |
pdrive5s-hnphs
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pDRIVE5Lucia-hNphsI pDRIVE bearing human NphsI promoter - Lucia |
Show product |
20 µg |
pdrive5lc-hnphs
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NphsI promoter (Podocytes) in pDRIVE expression plasmid
Nephrin is a transmembrane protein that belongs to the immunoglobulin superfamily and is localized to the slit diaphragms that are a critical component of the glomerular filtration barrier in the kidney.
A 1.25 kb fragment of the nephrin gene promoter, which includes the initiation codon and immediate 5’-flanking region, is capable of directing podocyte-specific expression of a β-galactosidase transgene in vivo [1,2].
This promoter lacks a TATA box but contains GATA binding sites and E-box consensus sequences. An enhancer was identified within the proximal promoter which is activated by the Wilms tumor suppressor protein (WT1) in vitro [3].
References:
1. Wong MA. et al., 2000. Identification and characterization of a glomerular-specific promoter from the human nephrin gene. Am J Physiol Renal Physiol. 279(6):F1027-32.
2. Moeller MJ. et al., 2002. Two gene fragments that direct podocyte-specific expression in transgenic mice. J Am Soc Nephrol. 13(6):1561-7.
3. Guo G. et al., 2004. WT1 activates a glomerular-specific enhancer identified from the human nephrin gene. J Am Soc Nephrol. 15(11):2851-6.
Specifications
pDRIVE bearing human Nphs1 promoter - LacZ, SEAP or Lucia
Gene Promoter: Nphs1
Specificity: Podocytes
Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia
Selection: Zeocin™
Back to the toppDRIVE contents
- 20 µg of lyophilized DNA
- 1 ml of Zeocin™ (100 mg/ml)
Product is shipped at room temperature
