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pDRIVE-NphsI

Product Unit size Cat. code Docs. Qty. Price

pDRIVE-hNphsI

pDRIVE bearing human NphsI promoter - LacZ

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20 µg

pdrive-hnphs
+-
$554

pDRIVE5SEAP-hNphsI

pDRIVE bearing human NphsI promoter - SEAP

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20 µg

pdrive5s-hnphs
+-
$554

pDRIVE5Lucia-hNphsI

pDRIVE bearing human NphsI promoter - Lucia

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20 µg

pdrive5lc-hnphs
+-
$554

NphsI promoter (Podocytes) in pDRIVE expression plasmid

Nephrin is a transmembrane protein that belongs to the immunoglobulin superfamily and is localized to the slit diaphragms that are a critical component of the glomerular filtration barrier in the kidney.

A 1.25 kb fragment of the nephrin gene promoter, which includes the initiation codon and immediate 5’-flanking region, is capable of directing podocyte-specific expression of a β-galactosidase transgene in vivo [1,2].

This promoter lacks a TATA box but contains GATA binding sites and E-box consensus sequences. An enhancer was identified within the proximal promoter which is activated by the Wilms tumor suppressor protein (WT1) in vitro [3].

 

References:

1. Wong MA. et al., 2000. Identification and characterization of a glomerular-specific promoter from the human nephrin gene. Am J Physiol Renal Physiol. 279(6):F1027-32.
2. Moeller MJ. et al., 2002. Two gene fragments that direct podocyte-specific expression in transgenic mice. J Am Soc Nephrol. 13(6):1561-7.
3. Guo G. et al., 2004. WT1 activates a glomerular-specific enhancer identified from the human nephrin gene. J Am Soc Nephrol. 15(11):2851-6.

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Specifications

pDRIVE bearing human Nphs1 promoter - LacZ, SEAP or Lucia

Gene Promoter: Nphs1

Specificity: Podocytes

Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia

Selection: Zeocin™

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pDRIVE contents

  • 20 µg of lyophilized DNA
  • 1 ml of Zeocin™ (100 mg/ml)

room temperature Product is shipped at room temperature

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