NF-κB Reporter THP-1 Cells

NF-κB–SEAP Reporter Monocytes

THP1-Blue™ NF-κB cells were specifically designed for monitoring the NF-κB signal transduction pathway in a physiologically relevant cell line. THP1-Blue™ cells were derived from the human THP-1 monocyte cell line by stable integration of an NF-κB-inducible SEAP reporter construct.

As a result, THP1-Blue™ NF-κB cells allow the monitoring of NF-κB activation by assessing the activity of SEAP (secreted embryonic alkaline phosphatase). The level of NF-κB-induced SEAP in the cell culture supernatant is readily assessed with QUANTI-Blue™, a SEAP detection reagent.

THP-1 cells endogenously express many pattern-recognition receptors (PRRs), including Toll-like receptors (TLRs), the stimulator of interferon genes (STING), and RIG-I-like receptors (RLR). Therefore, THP1-Blue™ NF-κB cells are highly responsive to PRR agonists that trigger the NF-κB pathway (see figure).

Key features

• Functionally validated with a selection of PRR ligands and cytokines
• Readily assessable NF-κB activation by assessing the SEAP activity

Note: A new clone is provided with an improved reporter/background signal ratio. The cat code has been changed accordingly (thp-nfkbv2).