Control Reporter THP-1 Cells - THP1-Null Cells

Control monocytes

ABOUT

Positive control cell line for inflammasome studies

THP1-Null cells are derived from THP-1 human monocytic cells, which represent the most commonly used model cell line for the study of inflammasome activation as they express high levels of NLRP3, ASC, and pro-caspase-1.

More details More details on THP1-Null cells

 

THP1-Null cells produce IL-1β upon stimulation with inflammasome inducers, such as MSU crystals and ATP.

As THP1-Null cells are fully efficient for NLRP3, Caspase 1 and ASC activities, they are the positive control cell line for THP1-defCASP1 Cells, which are deficient in Caspase 1.

 

Learn moreDownload our Practical Guide on Inflammasomes

Disclaimer:  These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.

SPECIFICATIONS

Specifications

Species
Human
Tested applications

Activated by inflammasome inducers (MSU crystals and ATP)

Cell type
Monocytic
Growth properties
Suspension
Tissue origin
Human monocytes
Antibiotic resistance
Hygromycin
Growth medium

Complete RPMI 1640 (see TDS)

Mycoplasma-free

Verified using Plasmotest™

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    THP1-Null Cells
  • Cat code: 
    thp-null
  • Quantity: 
    3-7 x 10^6 cells
Includes:
  • 1 ml of Hygromycin B Gold (100 mg/ml)
  • 1 ml of Normocin™ (50 mg/ml)

Shipping & Storage

  • Shipping method:  Dry ice
  • Storage:

    • Liquid nitrogen vapor
    Stability: 20 passages

    Caution:

    • Upon receipt, store immediately in liquid nitrogen vapor. Do not store cell vials at -80°C.

Details

THP1-Null cells are designed to study the signals involved in inflammasome activation. To become susceptible to inflammasome inducers, these cells must be induced by stimuli commonly used for induction in model systems, such as lipopolysaccharide (LPS) and phorbol 12-myristate acetate (PMA). Stimulation by LPS or differentiation with PMA induces the production of pro-IL-1β, the immature form of IL-1β. Subsequent stimulation with inflammasome inducers, such as ATP and alum, leads to caspase-1 activation and IL-1β maturation and secretion. Mature IL-1β can be detected by Western blot, ELISA, or a cell-based assay.

InvivoGen has developed a new method to detect bioactive IL-1β, based on HEK293 cells specifically engineered to selectively respond to IL-1β, named HEK-Blue™ IL-1β. These cells feature the SEAP (secreted embryonic alkaline phosphotase) reporter gene under the control of an NF-kB-inducible promoter. They naturally express the IL-1β receptor (IL-1R), and all the proteins involved in the MyD88-dependent IL-1R signaling pathway that leads to NF-kB activation. Thus upon IL-1β binding to IL-1R, a signaling cascade is initiated triggering NF-kB activation and the subsequent production of SEAP. Detection of SEAP in the supernatant of HEK-Blue™ IL-1β cells can be readily assessed using QUANTI-Blue™ Solution, a SEAP detection medium. QUANTI-Blue™ Solution turns blue in the presence of SEAP which can be easily quantified using a spectrophotometer.

DOCUMENTS

Documents

THP1-Null Cells

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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