Human IFI16-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

ABOUT

IFI16 knockout dual reporter monocytes

THP1-Dual™ KO-IFI16 cells were generated from THP1-Dual™ cells by stable knockout of the IFI16 (Interferon-γ-inducible protein 16) gene. IFI16 is an innate immune sensor with multiple functions; DNA sensor, regulator of interferon (IFN) expression, activator of the inflammasome, and regulator of genome function [1,2]. These cell lines were derived from human THP‑1 monocytes, a cell line often used to study DNA sensing pathways as they express all the cytosolic DNA sensors identified so far (with the exception of DAI).

THP1-Dual™ and THP1-Dual™ KO-IFI16 cells stably express two inducible secreted reporter genes: IFN-inducible Lucia luciferase and NF‑κB-inducible SEAP (secreted embryonic alkaline phosphatase). They can be used to study the role of IFI16 by monitoring IRF-induced Lucia luciferase activity, using QUANTI‑Luc™, a Lucia™ luciferase detection reagent.

THP1-Dual™ KO-IFI16 cells are resistant to the selectable markers blasticidin and Zeocin®.

 

References:

1. Jakobsen MR. & Paludan SR., 2014. IFI16: At the interphase between innate DNA sensing and genome regulation. Cytokine Growth Factor Rev. 25(6):649-55.
2. Unterholzner L. et al., 2010. IFI16 is an innate immune sensor for intracellular DNA. Nat Immunol. 11(11):997-1004.

Disclaimer:  These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.

SPECIFICATIONS

Specifications

Species
Human
Tested applications

Screening of PRR agonists or inhibitors

Cell type
Monocytic
Growth properties
Suspension
Tissue origin
Human monocytes
Reporter gene
SEAP
Lucia®
Detection method
Colorimetric / Luminescence
Antibiotic resistance
Blasticidin
Zeocin®
Growth medium

Complete RPMI 1640 (see TDS)

Mycoplasma-free

Validated using PlasmotestTM

Quality control

Each lot is functionally tested and validated

CONTENTS

Contents

  • Product: 
    THP1-Dual KO-IFI16 Cells
  • Cat code: 
    thpd-koifi16
  • Quantity: 
    3-7 x 10^6 cells
Includes:
  • 1 ml of Normocin™ (50 mg/ml)
  • 1 ml of Zeocin® (100 mg/ml)
  • 1 ml of Blasticidin (10 mg/ml)
  • 1 tube of QUANTI-Luc™ 4 Reagent
  • 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)

Shipping & Storage

  • Shipping method:  Dry ice
  • Storage:

    • Liquid nitrogen vapor
    Stability: 20 passages

DOCUMENTS

Documents

THP1-Dual KO-IFI16 Cells

Technical Data Sheet

Safety Data Sheet

Validation Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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