pFUSE-hIgG1e13-Fc
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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pFUSE-hIgG1e13-Fc1
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Show product |
20 µg |
pfc1-hg1e13
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pFUSE-hIgG1e13-Fc2
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Show product |
20 µg |
pfc2-hg1e13
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pFUSE-hIgG1e13-Fc1 is a cloning plasmid for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the human IgG1 heavy chain mutated I253A and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each part of the molecule to function independently.
pFUSE-hIgG1e13-Fc2 additionally features the IL2 signal sequence for the generation of Fc-Fusion proteins derived from proteins that are not naturally secreted.
Qiao S-W, et al. (2008). Dependence of antibody-mediated presentation of antigen on FcRn. PNAS 105(27): 9337-9342.
Strohl W.R. (2009). Optimization of Fc-mediated effector functions of monoclonal antibodies. Curr Opin Biotechnol 20: 685-691.
Petkova SB, et al. (2006). Enhanced half-life of genetically engineered human IgG1 antibodies in a humanized FcRn mouse model: potential application in humorally mediated autoimmune disease. Int Immunol 18(12):1759-69.
Specifications
Isotype: IgG1 human
Mutation: I253A
Characteristics:
- Decreased binding to FcRn
- Decreased half-life (enhanced antibody clearance)
Both plasmids are selectable with Zeocin™.
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- 20 µg of lyophilized DNA
- 1 ml of Zeocin® (100 mg/ml)
Product is shipped at room temperature