pFUSE-hIgG1e13-Fc1
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Cat.code:
pfc1-hg1e13
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ABOUT
pFUSE-hIgG1e13-Fc1 is a cloning plasmid for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the human IgG1 heavy chain mutated I253A and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each part of the molecule to function independently.
pFUSE-hIgG1e13-Fc2 additionally features the IL2 signal sequence for the generation of Fc-Fusion proteins derived from proteins that are not naturally secreted.
References
Qiao S-W, et al. (2008). Dependence of antibody-mediated presentation of antigen on FcRn. PNAS 105(27): 9337-9342.
Strohl W.R. (2009). Optimization of Fc-mediated effector functions of monoclonal antibodies. Curr Opin Biotechnol 20: 685-691.
Petkova SB, et al. (2006). Enhanced half-life of genetically engineered human IgG1 antibodies in a humanized FcRn mouse model: potential application in humorally mediated autoimmune disease. Int Immunol 18(12):1759-69.
All products are for research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
In vitro transfection, Fc-fusion protein generation
In vitro transfection
Plasmid construct is confirmed by restriction analysis and full-length open reading frame (ORF) sequencing.
Mutation: I253A
Characteristics:
- Decreased binding to FcRn
- Decreased half-life (enhanced antibody clearance)
CONTENTS
Contents
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Product:pFUSE-hIgG1e13-Fc1
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Cat code:pfc1-hg1e13
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Quantity:20 µg
1 ml of Zeocin® (100 mg/ml)
Shipping & Storage
- Shipping method: Room temperature
- Upon receipt, store product at -20 °C.
Storage:
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Plasmid Sequence
Certificate of analysis
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