Fc-mDectin-1a

Soluble murine Dectin-1 receptor

ABOUT

Soluble murine Dectin-1 receptor

Fc-mDectin-1a is a soluble murine Dectin-1 receptor constructed by fusing the C-terminal extracellular domain of mouse Dectin-1a (aa 67-244) to the C-terminus of an engineered human IgG1 Fc domain with a 10 amino acid linker.

Fc-mDectin-1a has an apparent molecular weight of ~55 kDa on SDS-PAGE.

Fc-mDectin-1a is expressed in CHO cells and purified by protein G affinity chromatography.

All InvivoGen products are for internal research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Source
CHO cells
Molecular weight
~55 kDa (SDS-PAGE)
Tag
Fc, N-ter
Formulation buffer

Phosphate buffer, Glycine, Saccharose, Stabilizing agents

Tested applications

Receptor binding assays

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    Fc-mDectin-1a
  • Cat code: 
    fc-mdec1a-2
  • Quantity: 
    2 x 50 µg
Includes:

1.5 ml endotoxin-free water

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

b-Glucan receptor binding assay

β-Glucan receptor binding assay:
96-well plates were coated with 0.5 µg of various β-glucans and incubated with 1 μg/ml Fc-hDectin-1a or Fc-mDectin-1a.
After 2 hours, an anti-IgG secondary antibody conjugated to alkaline phosphatase was added.
β-Glucan-Dectin-1 binding was assessed using the QUANTI-Blue™ assay.

DOCUMENTS

Documents

Fc-mDectin-1a

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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