
Fc-mDectin-1a
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Cat.code:
fc-mdec1a-2
- Documents
ABOUT
Soluble murine Dectin-1 receptor
Fc-mDectin-1a is a soluble murine Dectin-1 receptor constructed by fusing the C-terminal extracellular domain of mouse Dectin-1a (aa 67-244) to the C-terminus of an engineered human IgG1 Fc domain with a 10 amino acid linker.
Fc-mDectin-1a has an apparent molecular weight of ~55 kDa on SDS-PAGE.
Fc-mDectin-1a is expressed in CHO cells and purified by protein G affinity chromatography.
All InvivoGen products are for internal research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
Phosphate buffer, Glycine, Saccharose, Stabilizing agents
Receptor binding assays
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:Fc-mDectin-1a
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Cat code:fc-mdec1a-2
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Quantity:2 x 50 µg
1.5 ml endotoxin-free water
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
Details
β-Glucan receptor binding assay:
96-well plates were coated with 0.5 µg of various β-glucans and incubated with 1 μg/ml Fc-hDectin-1a or Fc-mDectin-1a.
After 2 hours, an anti-IgG secondary antibody conjugated to alkaline phosphatase was added.
β-Glucan-Dectin-1 binding was assessed using the QUANTI-Blue™ assay.
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
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