Recombinant Human ICOS-Fc Fusion Protein

Soluble human ICOS (CD278) fused to an IgG1 Fc domain

ABOUT

Soluble human ICOS (CD278) fused to an IgG1 Fc domain

Protein description

InvivoGen offers hICOS-Fc, a soluble human ICOS (CD278) chimera protein generated by fusing the N-terminal extracellular domain of human ICOS (aa 21-141) to the N-terminus of a human IgG1 Fc domain with a TEV (Tobacco Etch Virus) sequence linker.
hICOS-Fc has been produced in CHO cells and purified by affinity chromatography. It has an apparent molecular weight of ~47 kDa on an SDS‑PAGE gel.

 

ICOS background

Inducible Co-Stimulator (ICOS), also known as cluster of differentiation 278 (CD278) is an immunostimulatory immune checkpoint and a member of the CD28 superfamily. This Type I transmembrane protein is rapidly induced in CD4+ and CD8+ T cells upon activation. Its ligand, ICOS-L (CD275), is mostly expressed by antigen-presenting cells [1].

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Applications

  • Screening of high-affinity anti-human ICOS monoclonal antibodies by ELISA
  • Screening of anti-human ICOS-L monoclonal antibodies using competition assays

Quality control

  • Size and purity confirmed by SDS-PAGE
  • Protein validated by ELISA using an anti-hICOS monoclonal antibody
  • Protein validated by flow cytometry using Raji-hICOS-L cells

 

Reference

1. Amatore F et al. 2020. Role of ICOS in cancer immunotherapy. Expert Opin Biol Ther 20:141-150.

All InvivoGen products are for internal research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Source
CHO cells
Species
Human
Accession sequence

XP_047299978.1

Protein size
365 a.a. (secreted form)
Molecular weight
~ 47 kDa (SDS-PAGE)
Tag
Fc, C-ter
Purity
>98% (SDS-PAGE)
Formulation buffer

Sodium phosphate buffer with glycine, saccharose and stabilizing agents

Appearance (form)
Lyophilized pellet
Appearance (color)
White
Reconstitution buffer
Endotoxin-free water (provided)
Tested applications

ELISA, flow cytometry, cellular assays

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    hICOS-Fc
  • Cat code: 
    fc-hicos
  • Quantity: 
    50 µg
Includes:

1.5 ml of endotoxin-free water

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: -20°C for up to 1 year

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

Inducible Co-Stimulator (ICOS), also known as cluster of differentiation 278 (CD278) is an immunostimulatory immune checkpoint and a member of the CD28 superfamily. This transmembrane protein is rapidly induced in CD4+ and CD8+ T cells upon activation. Its ligand, ICOS-L (CD275), is mostly expressed by antigen-presenting cells [1]. The interaction between ICOS and ICOS-L delivers a secondary co-stimulatory signal through the activation of the transcription factor AKT, which promotes T cell proliferation and differentiation as well as the production of cytokines [1]. In tumor immunity, ICOS is involved in the amplification of the anti-tumor cytotoxic CD8+ T cell response, as well as the 'pro-tumor' function and maintenance of regulatory T cells (Tregs). Therefore, both agonistic and antagonistic monoclonal antibodies (mAbs) targeting this pathway are being investigated in combinational cancer immunotherapy [2]. Notably, ICOS agonistic mAbs have been shown to potentiate the effects of anti-CTLA4 mAbs [3]. 

 

References:

1. Amatore, F. et al. 2020. Role of ICOS in cancer immunotherapy. Expert Opin Biol Ther 20, 141-150.
2. Solinas, C. et al. 2020. The rationale behind targeting the ICOS-ICOS ligand costimulatory pathway in cancer immunotherapy. ESMO Open 5.
3. Soldevilla, M.M. et al. 2019. ICOS Costimulation at the Tumor Site in Combination with CTLA-4 Blockade Therapy Elicits Strong Tumor Immunity. Mol Ther 27, 1878-1891.

DOCUMENTS

Documents

hICOS-Fc

Technical Data Sheet

Safety Data Sheet

Validation Data Sheet

Certificate of analysis

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