hPD1-Fc
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Cat.code:
fc-hpd1
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ABOUT
Soluble human PD-1 (CD279) fused to an IgG1 Fc domain
Protein description
InvivoGen offers hPD1-Fc, a soluble human PD-1 (CD279) chimera protein generated by fusing the N-terminal extracellular domain of human PD-1 (aa 24-167) to the N-terminus of a human IgG1 Fc domain with a TEV (Tobacco Etch Virus) sequence linker.
hPD1-Fc has been produced in CHO cells and purified by affinity chromatography. It has an apparent molecular weight of ~62 kDa on an SDS‑PAGE gel.
PD-1 background
Programmed cell death 1 (PD-1), also known as cluster of differentiation 279 (CD279) is a Type I transmembrane protein expressed at the cell surface of activated and exhausted conventional T cells. PD-1 is an inhibitory immune checkpoint that prevents T cell overstimulation and host damage. PD-1 interaction with its ligands PD-L1 and PD-L2 induces inhibition of TCR signaling [1].
Applications
- Screening of high-affinity anti-human PD-1 monoclonal antibodies by ELISA
- Screening of anti-human PD-L1 monoclonal antibodies using competition assays
Quality control
- Size and purity confirmed by SDS PAGE
- Protein validated by ELISA using Anti-hPD1-Ni-hIgG1 and Anti-hPD1-Pem-hIgG1 mAbs
- Protein validated by FACS using Raji-APC-hPD-L1 cells
References
1. Juneja, V.R. et al. 2017. PD-L1 on tumor cells is sufficient for immune evasion in immunogenic tumors and inhibits CD8 T cell cytotoxicity. J Exp Med 214, 895-904.
2. Kythreotou, A. et al. 2018. PD-L1. J Clin Pathol 71, 189-194.
All InvivoGen products are for internal research use only, and not for human or veterinary use.
InvivoGen also offers:
SPECIFICATIONS
Specifications
XP_006712636.1
Sodium phosphate buffer with glycine, saccharose, and stabilizing agents
ELISA, flow cytometry
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:hPD1-Fc
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Cat code:fc-hpd1
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Quantity:50 µg
1.5 ml endotoxin-free water
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
Details
Immune checkpoint signal: PD-1 and PD-L1
— PD-1 (programmed cell death 1; also known as CD279) is a type I transmembrane protein expressed at the cell surface of activated and exhausted conventional T cells. PD-1 is an inhibitory immune checkpoint that prevents T-cell overstimulation and host damage. PD-1 interaction with its ligands PD-L1 and PD-L2 induces inhibition of TCR signaling [1].
— PD-L1 (programmed cell death ligand 1; also known as CD274 or B7-H1) is a transmembrane protein expressed at the cell surface of hematopoietic and non-hematopoietic cells and is induced by pro-inflammatory cytokines, such as in the tumor microenvironment [1]. PD-L1 is one ligand for PD-1, an inhibitory immune checkpoint receptor that is expressed by activated and exhausted T cells. PD-1:PD-L1 interaction induces inhibition of TCR signaling, thereby preventing T-cell overstimulation and host damage [1].
PD-L1 expression is an immune evasion mechanism exploited by various malignancies and is generally associated with poorer prognosis [2]. Specifically, over-expressed PD-L1 on tumor cells and tumor-infiltrating immune cells, such as macrophages, can bind to PD-1 on cytotoxic T cells, and ultimately inhibit the anti-tumor T cell response [3, 4]. Thus, due to PD-L1’s instrumental role in immune evasion by cancer cells, there are numerous inhibitors in development as promising immuno-oncology therapies. Notably, Atezolizumab (also known as MPDL3280A), a fully humanized IgG1 (N298A) mAb that blocks the interaction of PD-L1 with PD-1 and induces anti-tumor immune reactivation, has been approved by the FDA for combinational use in the treatment of lung and breast cancer [3, 5].
References
1. Ribas A. and Wolchock J.D., 2018. Cancer immunotherapy using checkpoint blockade. Science. 359:1350-55.
2. Sun, C. et al. 2018. Regulation and Function of the PD-L1 Checkpoint. Immunity 48, 434-452.
3. Kythreotou, A. et al. 2018. PD-L1. J Clin Pathol 71, 189-194.
4. Lau, J. et al. 2017. Tumour and host cell PD-L1 is required to mediate suppression of anti-tumour immunity in mice. Nat Commun 8, 14572.
5. Heimes, A.S. & Schmidt, M. 2019. Atezolizumab for the treatment of triple-negative breast cancer. Expert Opin Investig Drugs 28, 1-5.
DOCUMENTS
Documents
Technical Data Sheet
Validation Data Sheet
Safety Data Sheet
Certificate of analysis
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