Mouse STING ISG Dual Reporter HEK 293 Cells

293-Dual™ mSTING Cells (ISG-SEAP/KI-[IFN-β]Lucia)

293-Dual™ mSTING (ISG/KI-IFNb) cells were generated from 293-Dual™ Null (ISG/KI-IFNb) cells by stable transfection of the murine STING gene.

These cells exhibit a robust response to STING agonists such as 2’3’-cGAMP and 3’3’-cGAMP. In addition, unlike cells with human STING, these cells respond to DMXAA (5,6-Dimethylxanthenone 4-acetic acid) which induces a potent IFN response by activating murine STING[2, 3].

293-Dual™ mSTING (ISG/KI-IFNb) cells are resistant to blasticidin, hygromycin and Zeocin®.

References:

1. Yi G. et al., 2013. Single nucleotide polymorphisms of human STING can affect innate immune response to cyclic dinucleotides. PLoS One. 8(10):e77846.
2. Conlon J. et al., 2013. Mouse, but not human STING, binds and signals in response to the vascular disrupting agent 5,6-dimethylxanthenone-4-acetic acid. J Immunol 190(10):5216-25.
3. Zhang H. et al., 2015. Rat and human STINGs profile similarly towards anticancer/antiviral compounds. Sci Rep. 5:18035.