Human STING (R232) ISG Dual Reporter HEK 293 Cells

293-Dual™ hSTING-R232 Cells (ISG-SEAP/KI-[IFN-β]Lucia)

293-Dual™ hSTING-R232 (ISG/KI-IFNb) cells were generated from 293-Dual™ Null (ISG/KI-IFNb) cells by stable transfection of the R232 isoform of human STING (hSTING). These cells allow a dual monitoring of the ISG/IFN pathway as they express two different reporter constructs, featuring the secreted embryonic alkaline phosphatase (SEAP) and Lucia® (encoding a secreted luciferase) reporter genes. Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™ Solution, a SEAP detection reagent, and QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia and Gaussia luciferase detection reagent. 

Genomic studies indicate that this isoform, which contains an arginine at position 232 (R232), is the most prevalent with an isoform occurrence of ~60% in the human population [1, 2]. This isoform is preferentially activated by 2’5’linkage-containing cGAMP isomers [3].

References:

1. Yi G. et al., 2013. Single nucleotide polymorphisms of human STING can affect innate immune response to cyclic dinucleotides. PLoS One. 8(10):e77846.
2. Jin L. et al., 2011. Identification and characterization of a loss-of-function human MPYS variant. Genes Immun. 12(4):263-9.
3. Zhang X. et al., 2013. Cyclic GMP-AMP containing mixed phosphodiester linkages is an endogenous high-affinity ligand for STING. Mol. Cell. 51:226–235.