CD 14
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
|---|---|---|---|---|---|---|
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pUNO1-hCD14 pUNO1 bearing the human CD14 gene |
Show product |
20 µg |
puno1-hcd14
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pUNO1-mCD14 pUNO1 bearing the mouse CD14 gene |
Show product |
20 µg |
puno1-mcd14
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Expression-ready ORF Clones
CD14 molecule.
Membrane-bound form
CD14 is a glycosylphosphatidylinositol (GPI)-anchored membrane protein which acts as a bacterial pattern recognition receptor [1]. CD14 is found on cells derived from the monocyte/macrophage lineage, as well as neutrophils and B lymphocytes. CD14 serves as a member of the heteromeric lipopolysaccharide (LPS) receptor complex that also contains TLR4 and MD2 [2]. CD14 binds LPS but is not capable of initiating a transmembrane activation signal since it does not contain a cytoplasmic domain. Upon LPS binding, CD14 physically associates with TLR4 which in turn transduces the signal. CD14 was also shown to interact with TLR2 in response to various microbial infections [3].
1. Pugin J. et al., 1994. CD14 is a pattern recognition receptor. Immunity, 1(6):509-16.
2. Da Silva Correia J. et al., 2001. Lipopolysaccharide is in close proximity to each of the proteins in its membrane receptor complex. transfer from CD14 to TLR4 and MD-2. J Biol Chem, 276(24): 21129-35.
3. Aderem A. & R.J. Ulevitch, 2000. Toll-like receptors in the induction of the innate immune response. Nature, 406(6797):782-7.
Specifications
Human CD14 (pUNO1-hCD14)
- ORF size : 1128 bp
- Subclone : NcoI - EcoRI
Mouse CD14 (pUNO1-mCD14)
- ORF size : 1101 bp
- Subclone : NcoI - NheI
pUNO1 Contents
- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
Product is shipped at room temperature.
Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.
Resuspended DNA should be stored at -20°C and is stable for up to 1 year.
Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.