cGAS
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
|---|---|---|---|---|---|---|
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pUNO1-hCGAS pUNO1 bearing the human cGAS gene |
Show product |
20 µg |
puno1-hcgas
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pUNO1-hCGAS-HA3x pUNO1 bearing the human cGAS-HA gene |
Show product |
20 µg |
puno1ha-hcgas
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pUNO1-hCGAS-AA pUNO1 bearing the human cGAS gene, G212A+S213A inactive mutant |
Show product |
20 µg |
puno1-hcgas-aa
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pUNO1-mCGAS pUNO1 bearing the mouse cGAS gene |
Show product |
20 µg |
puno1-mcgas
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pUNO1-mCGAS-HA3x pUNO1 bearing the mouse cGAS-HA gene |
Show product |
20 µg |
puno1ha-mcgas
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pUNO1-mCGAS-AA pUNO1 bearing the mouse cGAS gene, G212A+S213A inactive mutant |
Show product |
20 µg |
puno1-mcgas-aa
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Expression-ready ORF Clones
Alias : MB21D1, CGAS
Cyclic-GMP/AMP synthase.
Mab-21 domain containing 1.
cGAS::HA3x :
The cGAS gene has been fused at the 3'end to the influenza hemaglutinine (HA) tag. This short sequence (YPYDVPDYA) encodes a peptide which is the epitope of a very efficient and specific monoclonal antibody. The use of HA-tagged genes provides a simple and convenient method to detect the expression of the genes by Western blot.
Loss of function cGAS mutants :
Two point mutations have been introduced in cGAS: G212A and S213A. These mutations in residues of the NTase active site abolish the activity of full-length cGAS [1,2].
1. Civril F, et al. (2013) Nature 498(7454):332-7
2. Marcus A, et al. (2018) Immunity. 49(4):754-763.e4
Specifications
Human cGAS (pUNO1-hCGAS)
Genbank : NM_138441.3 with one silent mutation in codon Lys500
ORF size : 1569 bp
Subclone : AgeI - AvrII
Mouse cGAS (pUNO1-mCGAS)
Genbank : KC294567.1
ORF size : 1524 bp
Subclone : AgeI - NheI
Human cGAS-HA (pUNO1-hcGAS-HA3x)
ORF size : 1662 bp
Subclone : AgeI - MscI
Mouse cGAS-HA (pUNO1-mcGAS-HA3x)
ORF size : 1617 bp
Subclone : AgeI - NheI
pUNO1 Contents
- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
Product is shipped at room temperature.
Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.
Resuspended DNA should be stored at -20°C and is stable for up to 1 year.
Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.