RIPK1

Expression-ready ORF Clones

Alias : RIP1

Receptor interacting serine/threonine kinase 1.

Receptor-interacting protein (RIP1), a serine/threonine kinase, mediates the activation of NF-kB in response to TNF [1]. RIP1 belongs to a family of related kinases that includes RIP2 and RIP3 that share significant homology in their N termini but possess distinct C termini [2]. RIP1 contains a death domain (DD) and interacts with proteins containing DD such as TNFR1, Fas, TRAIL and FADD [3]. RIP1 was shown to be an essential mediator of TLR3-induced NF-kB activation [4]. TLR3 signals through TRIF which recruits RIP1 which in turn associates with IKK leading to the activation of NF-kB.

References

1. Kelliher MA. et al., 1998. The death domain kinase RIP mediates the TNF-induced NF-kappaB signal. Immunity. 8(3):297-303.
2. Sun X. et al., 1999. RIP3, a novel apoptosis-inducing kinase. J Biol Chem. 274(24):16871-5.
3. Lin Y. et al., 2000. The death domain kinase RIP is essential for TRAIL (Apo2L)-induced activation of IkappaB kinase and c-Jun N-terminal kinase.Mol Cell Biol. 20(18):6638-45.
4. Meylan E. et al., 2004. RIP1 is an essential mediator of Toll-like receptor 3-induced NF-kappa B activation. Nat Immunol. 5(5):503-7.

Specifications

Human RIPK1 (pUNO1-hRIPK1)

Genbank : NM_003804.3
ORF size : 2016 bp
Subclone : AgeI - NheI

Mouse RIPK1 (pUNO1-mRIPK1)

ORF size : 1971 bp
Subclone : AgeI - NheI

pUNO1 Contents

• 20 µg of lyophilized DNA
• 2 x 1 ml blasticidin at 10 mg/ml

Product is shipped at room temperature.

Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.

Resuspended DNA should be stored at -20°C and is stable for up to 1 year.

Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.