RIPK3
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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pUNO1-hRIPK3 pUNO1 bearing the human RIPK3 gene |
Show product |
20 µg |
puno1-hripk3
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pUNO1-mRIPK3a pUNO1 bearing the mouse RIPK3 gene, isoform 1 |
Show product |
20 µg |
puno1-mripk3
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Expression-ready ORF Clones
Alias : RIP3
Receptor interacting serine/threonine kinase 3.
Receptor-interacting protein 3 (RIP3) is a member of the RIP Ser/Thr kinase family [1]. Unlike RIP1 and RIP2, RIP3 possesses neither a death domain nor a CARD motif at its unique C terminus [2]. RIP3 is a potent inducer of apoptosis, an activity readily localizable to its C terminus. RIP3 binds RIP within the TNFR-1 signaling complex and attenuates its ability to engage the NF-kB pathway [2,3]. Upon TNF stimulation, RIP1 is recruited to the TNFR1 signaling complex to activate NF-kB. RIP3 is then recruited and phosphorylates RIP1 inhibiting its ability to activate NF-kB. Thus RIP3 acts as a modulator that can limit persistent activation of the NF-kB pathway [3].
References:
1. Yu PW. et al., 1999. Identification of RIP3, a RIP-like kinase that activates apoptosis and NFkappaB. Curr Biol. 9(10):539-42.
2. Sun X. et al., 1999. RIP3, a novel apoptosis-inducing kinase. J Biol Chem. 274(24):16871-5.
3.Sun X. et al., 2002. Identification of a novel homotypic interaction motif required for the phosphorylation of receptor-interacting protein (RIP) by RIP3. J Biol Chem. 277(11):9505-11.
Specifications
Human RIPK3 (pUNO1-hRIPK3)
Genbank : NM_006871.3
ORF size : 1557 bp
Subclone : BspLU11I - NheI
Mouse RIPK3 isoform 1 (pUNO1-mRIPK3a)
Genbank : NM_019955.2 with silent variation in codon Val291.
ORF size : 1461 bp
Subclone : AgeI - EcoRI
pUNO1 Contents
- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
Product is shipped at room temperature.
Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.
Resuspended DNA should be stored at -20°C and is stable for up to 1 year.
Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.