pDRIVE-HSP70
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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pDRIVE-hHSP70 pDRIVE bearing human HSP70 promoter - LacZ |
Show product |
20 µg |
pdrive-hhsp70
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pDRIVE5SEAP-hHSP70 pDRIVE bearing human HSP70 promoter - SEAP |
Show product |
20 µg |
pdrive5s-hhsp70
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pDRIVE5Lucia-hHSP70 pDRIVE bearing human HSP70 promoter - Lucia |
Show product |
20 µg |
pdrive5lc-hhsp70
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pDRIVE-mHSP70 pDRIVE bearing mouse HSP70 promoter - LacZ |
Show product |
20 µg |
pdrive-mhsp70
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pDRIVE5SEAP-mHSP70 pDRIVE bearing mouse HSP70 promoter - SEAP |
Show product |
20 µg |
pdrive5s-mhsp70
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pDRIVE5Lucia-mHSP70 pDRIVE bearing mouse HSP70 promoter - Lucia |
Show product |
20 µg |
pdrive5lc-mhsp70
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HSP70 promoter (Ubiquitous, heat-inducible) in pDRIVE expression plasmid
Heat inducible
The HSP70 gene encodes a major stress-inducible heat shock protein (HSP70) which plays an important role in protecting cells from deleterious stresses. Hsp70 is expressed constitutively in a wide variety of cells at normal growth temperatures.
Its expression is induced by various physiological stresses, including exposure to heat shock and heavy metal, or under non-stress conditions, such as in response to serum stimulation.
The HSP70 promoter contains at least two regulatory domains, a distal domain responsive to heat shock or heavy metals and a proximal domain responsive to stimulation by serum [1].
The activity of the HSP70 promoter can be induced by moderate hyperthermia (39°C to 43°C) [2], reaching expression levels similar to those of the CMV promoter.
References:
1. Wu BJ. Kingston RE, Morimoto RI. 1986. Human HSP70 promoter contains at least two distinct regulatory domains. Proc Natl Acad Sci USA. 83(3):629-33.
2. Huang Q. et al. 2000. Heat-induced gene expression as a novel targeted cancer gene therapy strategy. Cancer Res. 60(13):3435-9.
Specifications
pDRIVE bearing human HSP70 promoter - LacZ, SEAP or Lucia
Gene Promoter: Heat Shock Protein 70
Specificity: Ubiquitous, heat inducible
Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia
Selection: Zeocin™
pDRIVE bearing mouse HSP70 promoter - LacZ, SEAP or Lucia
Gene: Heat Shock Protein 70
Specificity: Ubiquitous, heat inducible
Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia
Selection: Zeocin™
Back to the toppDRIVE contents
- 20 µg of lyophilized DNA
- 1 ml of Zeocin™ (100 mg/ml)
Product is shipped at room temperature
