ODN BW006 (ODN 684)
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ODN BW006 (ODN 684) Class B |
Show product |
200 µg |
tlrl-bw006
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Product not available in China. For more information, please contact InvivoGen Asia.
Class B CpG oligonucleotide - Human & mouse TLR9 ligand
ODN BW006 (also known as ODN 684) is a synthetic oligonucleotide that contains unmethylated CpG dinucleotides in particular sequence contexts (CpG motifs), that are recognized by TLR9 leading to strong immunostimulatory effects.
ODN BW006 is a type B CpG ODN containing twice the optimal motif in human, GTCGTT [1]. ODN BW006 induces the proliferation of human peripheral blood mononucleated cells (PBMCs), peripheral blood mononucleated cells, (PBMCs), and mouse splenocytes, as vigorously as ODN 2006, a class B prototype ODN.
It was found to improve the rabies vaccine by inducing an earlier and more vigorous protective response [1]. ODN BW006 promotes a strong Th1 response [2, 3].
References:
1. Wang X. et al., 2008. A CpG oligodeoxynucleotide acts as a potent adjuvant for inactivated rabies virus vaccine.Vaccine. 26(15):1893-901.
2. Yan Y. et al., 2012. A CpG oligodeoxynucleotide potentiates the anti-tumor effect of HSP65-Her2 fusion protein against Her2 positive B16 melanoma in mice. Int Immunopharmacol. 12(2):402-7.
3. Zhang X. et al., 2011. Enhanced specific immune responses by CpG DNA in mice immunized with recombinant hepatitis B surface antigen and HB vaccine. Virol J. 8:78.
Specifications
Specificity: human and murine TLR9 agonist
Working concentration: 1-5 μM OR 1-50 µg/ml
ODN BW006 sequence
5’-tcg acg ttc gtc gtt cgt cgt tc-3’ (23 mer)
Note: Bases are phosphorothioate (nuclease resistant).
Quality control:
- TLR9 activity has been tested using HEK-Blue™ TLR9 cells.
- The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.
Contents
- 200 μg (27.24 nmol) of ODN BW006 provided lyophilized
Note: ODN BW006 is sterile filtered prior to lyophilization.
- 1.5 ml endotoxin-free water
ODN BW006 is shipped at room temperature
ODN BW006 should be stored at -20°C.