MyD88
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pUNO1-hMYD88 pUNO1 bearing the human MyD88 gene |
Show product |
20 µg |
puno1-hmyd88
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pUNO1-hMyD88-DN pUNO1 bearing a dominant negative version of the human MyD88 gene |
Show product |
20 µg |
puno1-hmyd88-dn
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pUNO1-mMYD88 pUNO1 bearing the mouse MyD88 gene |
Show product |
20 µg |
puno1-mmyd88
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pUNO1-mMyD88-DN pUNO1 bearing a dominant negative version of the mouse MyD88 gene |
Show product |
20 µg |
puno1-mmyd88-dn
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Expression-ready ORF Clones
Myeloid Differentiation Primary Response 88.
Available versions:
- Wild type MyD88
- Dominant negative MyD88 (see the "Details" tab)
Myeloid differentiation factor 88 (MyD88) is a cytoplasmic adaptor protein that is involved in interleukin-1 receptor (IL-1R)- and Toll-like receptor (TLR)-induced activation of NF-kappaB. MyD88 contains a C-terminal Toll/IL-1R homology (TIR) domain and an N-terminal death domain (DD), which mediate the interaction of MyD88 with IL-1R/TLR and IRAK, respectively [1]. MyD88 is essential for signaling as MyD88 -/- mice fail to induce cytokines in response to LPS and CpG DNA [2]. Some TLRs induce antiviral responses by producing IFN-alpha in a MyD88-dependent manner. MyD88 interacts with IRF7 resulting in the activation of IFN-alpha-dependent promoters [3].
1. Janssens S. et al., 2002. Regulation of interleukin-1- and lipopolysaccharide-induced NF-kappaB activation by alternative splicing of MyD88. Curr Biol. 12(6):467-71.
2. Kawai T. et al. 1999. Unresponsiveness of MyD88-deficient mice to endotoxin. Immunity 11(1):115-22.
3. Kawai T. et al., 2004. Interferon-alpha induction through Toll-like receptors involves a direct interaction of IRF7 with MyD88 and TRAF6. Nat Immunol. 5(10):1061-8.
Specifications
Human MyD88 (pUNO1-hMYD88)
Genbank : NM_002468.3
ORF size : 891 bp
Subclone : NcoI - NheI
Human MyD88-DN (pUNO1-hMyD88-DN)
ORF size : 420 bp
Subclone : NcoI - NheI
Mouse MyD88 (pUNO1-mMYD88)
ORF size : 891 bp
Subclone : BspLU11I - NheI
Mouse MyD88-DN (pUNO1-mMyD88-DN)
ORF size : 420 bp
Subclone : NcoI - NheI
pUNO1 Contents
- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
Product is shipped at room temperature.
Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.
Resuspended DNA should be stored at -20°C and is stable for up to 1 year.
Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.