LY96
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pUNO1-bLY96 pUNO1 bearing the bovine LY96 (MD2) gene |
Show product |
20 µg |
puno1-bly96
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pUNO1-hLY96a pUNO1 bearing the human LY96 (MD2) gene, isoform 1 |
Show product |
20 µg |
puno1-hly96a
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pUNO1-mLY96a pUNO1 bearing the mouse LY96 (MD2) gene, isoform 1 |
Show product |
20 µg |
puno1-mly96a
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Expression-ready ORF Clones
Alias : MD2
Lymphocyte antigen 96.
Myeloid differentiation factor 2.
MD-2 (LY96), a 25-kDa secreted protein, is an accessory molecule required for efficient LPS-induced signaling through TLR4. MD-2 binds to both LPS and the extracellular domain of TLR4. Following synthesis, MD-2 is either secreted directly into the medium as a soluble, active protein, or binds directly to TLR4 in the endoplasmic reticulum before migrating to the cell surface. The secreted form of MD-2 forms a stable complex with LPS, in a CD14-dependent process [2]. The MD-2/LPS complex activates TLR4 in the absence of CD14 or free LPS. indicating that the activating ligand of TLR4 is the MD-2/LPS complex. MD-2 also increases TLR2 responsiveness to TLR2 ligands [3].
The pUNO1 plasmid bearing the human LY96 (MD2) gene, isoform 2, is available upon request. Contact us for more information.
1. Shimazu R. et al., 1999. MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4. J Exp Med, 189(11):1777-82.
2. Kennedy MN. et al., 2004. A complex of soluble MD-2 and lipopolysaccharide serves as an activating ligand for Toll-like receptor 4. J Biol Chem. 2004 Aug 13;279(33):34698-704.
3. Dziarski R. et al., 2001. MD-2 enables Toll-like receptor 2 (TLR2)-mediated responses to lipopolysaccharide and enhances TLR2-mediated responses to Gram-positive and Gram-negative bacteria and their cell wall components. J Immunol, 166(3):1938-44.
Specifications
Bovine LY96 (pUNO1-bLY96)
Genbank : NM_001046517.1
ORF size : 483 bp
Subclone : BspLU11I - NheI
Human LY96 isoform 1 (pUNO1-hLY96a)
Genbank : NM_015364.4
ORF size : 483 bp
Subclone : BspLU11I - NheI
Mouse LY96 isoform 1 (pUNO1-mLY96a)
Genbank : NM_016923.2
ORF size : 483 bp
Subclone : BspLU11I - NheI
pUNO1 Contents
- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
Product is shipped at room temperature.
Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.
Resuspended DNA should be stored at -20°C and is stable for up to 1 year.
Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.