B16-Blue™ ISG Cells
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Cat.code:
bb-ifnabg
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ABOUT
Interferon Regulatory Factor-Inducible SEAP Reporter B16 Melanocytes
B16-Blue™ ISG cells allow the detection of bioactive murine type I and II IFNs by monitoring the activation of the JAK/STAT pathway.
B16-Blue™ ISG cells can also be used to study the activation of the TBK1/IRF3 pathway by cytosolic DNA, dsRNA or CDNs.
B16-Blue™ ISG cells were derived from the murine B16 melanoma cell line. They express the secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the I-ISG54 promoter which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE.
Stimulation of B16- Blue™ ISG cells with IFNs, CDNs, such as cGAMP, or type I IFN inducers, such as transfected poly(dA:dT), triggers the activation of the I-ISG54 promoter and the production of SEAP.
Levels of SEAP in the supernatant can be easily determined using QUANTI- Blue™, a reagent that turns purple/blue in the presence of SEAP, by reading the OD at 620-655 nm.
B16-Blue™ ISG cells are resistant to Zeocin®.
These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.
SPECIFICATIONS
Specifications
Reporter activity is validated by stimulating the cells with murine IFN-α, mIFN-β, mIFN-γ, and IRF3 activators
- mIFN-α: 10e2 - 10e4 IU/ml
- mIFN-β: 10e2 - 10e4 IU/ml
- mIFN-γ: 0.1 ng - 1 µg/ml
Complete DMEM (see TDS)
Verified using PlasmotestTM
Each lot is functionally tested and validated
CONTENTS
Contents
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Product:B16-Blue™ ISG Cells
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Cat code:bb-ifnabg
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Quantity:3-7 x 10^6 cells
1 ml of Zeocin® (100 mg/ml)
1 ml of Normocin™ (50 mg/ml)
1 ml of QB reagent and 1 ml of QB buffer
Shipping & Storage
- Shipping method: Dry ice
- Liquid nitrogen
Storage:
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
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