Anti-dCD20c1-dIgG2

Monoclonal dog IgG2 antibody against dog CD20

ABOUT

Monoclonal dog IgG2 antibody against dog CD20

Anti-dCD20c1-dIgG2 is a chimeric monoclonal antibody specific for dog CD20 (dCD20). TIn flow cytometry applications, this antibody has exhibited weak cross-reactivity with human CD20. Reactivity with other species has not been tested.

It has been produced in CHO cells and purified by affinity chromatography with protein A.

Anti-dCD20c1-dIgG2 was generated by combining the constant domains of the dog IgG2 molecule with the variable regions of Anti-dCD20c1-mIgG2a.

Anti-dCD20c1-mIgG2a has been selected for its ability to bind dCD20 and induce complement-dependent cytotoxicity (CDC).

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Target

CD20

Target species

Dog

Applications

Flow cytometry, cell lysis, B-cell depletion Not suitable for immunohistochemical staining.

Isotype
dIgG2
kappa
Clone
6C12
Source
CHO cells
Purification
Protein A
Formulation buffer

68 mM phosphate buffer with 91 mM glycine, 5% w/v saccharose and stabilizing agents.

Preservative
Azide-free
Reconstitution buffer
Sterile water
Sterility

The absence of bacterial contamination (e.g. endotoxins and peptidoglycans) is confirmed by using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.

Tested applications

Flow cytometry, cell lysis, B-cell depletion. Not suitable for immunohistochemical staining.

Quality control

The absence of bacterial contamination (e.g. endotoxins and peptidoglycans) is confirmed by using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells. Recognition of dCD20 with this antibody is tested using flow cytometry.

CONTENTS

Contents

  • Product: 
    Anti-dCD20c1-dIgG2
  • Cat code: 
    dcd20-mab1
  • Quantity: 
    100 µg

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: Reconstituted antibody is stable for 1 month when stored at 4°C and 6 months when aliquoted and stored at -20°C.

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

Anti-dCD20c1-dIgG2

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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