NF-κB Reporter Ramos Cells (MyD88 deficient)

NF-κB/AP-1 SEAP reporter B lymphocytes with MyD88 knockdown

Ramos-Blue™ KD-MyD (MyD88 knockdown) is a B lymphocyte cell line specifically designed to monitor MyD88-mediated signaling.

Ramos-Blue™ KD-MyD were obtained by stable integration of an NF-κB/AP-1-inducible SEAP (secreted embryonic alkaline phosphate) reporter gene and stable knowndown of the MyD88 gene.

Due to the knockdown of the MyD88 gene, Ramos-Blue™ KD-MyD do not respond or respond weakly to the activation of receptors which signaling is dependent on MyD88, such as TLR7 and TLR9. However, they are still responsive to TLR3 which signals through TRIF independently of MyD88.

When stimulated, they produce SEAP in the supernatant that can be readily monitored using QUANTI-Blue™, a medium that turns purple/blue in the presence of SEAP.

Ramos-Blue™ KD-MyD cells derive from a human Burkitt’s lymphoma which is negative for Epstein Barr virus.