Pepinh-MYD

MyD88 Inhibitory Peptide

Pepinh-MYD is a 26 aa peptide that blocks MyD88 signaling by inhibiting its homodimerization through binding.

Pepinh-MYD contains a sequence from the MyD88 TIR homodimerization domain (RDVLPGT) [1] preceeded by a protein transduction sequence (RQIKIWFQNRRMKWKK) derived from antennapedia which enables the peptide to translocate through the cell membrane [2].

Pepinh-MYD is provided with Pepinh-Control, a control peptide [3].

References:

1. loiarro M. et al., 2005. Peptide-mediated Interference of TIR Domain Dimerization in MyD88 Inhibits Interleukin-1-dependent Activation of NF-{kappa}B. J. Biol. Chem., 280: 15809-14.
2. Derossi D. et al., 1994. The third helix of the Antennapedia homeodomain translocates through biological membranes. J. Biol. Chem., 269: 10444-50.
3. Toshchakov V. et al., 2005. Differential Involvement of BB Loops of Toll-IL-1 Resistance (TIR) Domain-Containing Adapter Proteins in TLR4- versus TLR2-Mediated Signal Transduction. J. Immunol., 175: 494 - 500.

Specifications

Working concentration: 5-50 μM

Purity:  ≥95% (UHPLC)

Species reactivity: Human, mouse

Sequences:

• Pepinh-MYD:    RQIKIWFQNRRMKWKK-RDVLPGTCVNS-NH2 (MyD88 homodimerization sequence is underlined)
• Pepinh-Control: RQIKIWFQNRRMKWKK-SLHGRGDPMEAFII-NH2

Molecular weights:

• Pepinh-MYD: 3430
• Pepinh-Control: 3812

Contents

Pepinh-MYD is provided as a white solid.

• 2 mg of lyophilized Pepinh-MYD
• 2 mg of lyophilized Pepinh-Control
• 2x1.5 ml endotoxin-free water

Pepinh-MYD is shipped at room temperature.

Store at -20°C.

Solid product is stable 1 year at -20°C.

Upon resuspension, Pepinh-MYD should be aliquoted and stored at -20°C.

Avoid repeated freeze-thaw cycles.

Resuspended product is stable for 6-8 months at -20°C when properly stored.