
pSELECT-hASC-GFP
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Cat.code:
psetz-hascgfp
- Documents
ABOUT
A mammalian expression plasmid encoding an ASC::GFP fusion protein
pSELECT-ASC-GFP are mammalian expression vectors designed for the expression of the human or murine ASC genes fused to the green fluorescent protein (GFP). These plasmids are selectable in bacteria and mammalian cells with Zeocin™.
pSELECT-ASC-GFP plasmids contain two expression cassettes. The first delivers strong and constitutive expression of hASC::GFP or mASC::GFP. The second drives the zeocin-resistance gene (Sh ble) that allows the selection of stable transfectants (and selection in bacteria). Both cassettes are terminated with a strong polyadenylation signal that separates the two expression cassettes thus preventing any transcription interference.
In transfected cells equipped with inflammasome components, ASC::GFP expression enables the visual monitoring of ASC specks, a hallmark of inflammasome activation .
The same plasmid is available with the GFP gene alone as a control (pSELECT-CGFP-zeo).
All products are for research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
Plasmid construct has been confirmed by restriction analysis and sequencing.
CONTENTS
Contents
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Product:pSELECT-hASC-GFP
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Cat code:psetz-hascgfp
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Quantity:20 µg
1 ml of Zeocin™ (100 mg/ml)
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
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