ASC-GFP

ASC::GFP fusion gene

ABOUT

A mammalian expression plasmid encoding an ASC::GFP fusion protein 

 

pSELECT-ASC-GFP are mammalian expression vectors designed for the expression of the human or murine ASC genes fused to the green fluorescent protein (GFP). These plasmids are selectable in bacteria and mammalian cells with Zeocin™.

pSELECT-ASC-GFP plasmids contain two expression cassettes. The first delivers strong and constitutive expression of hASC::GFP or mASC::GFP. The second drives the zeocin-resistance gene (Sh ble) that allows the selection of stable transfectants (and selection in bacteria). Both cassettes are terminated with a strong polyadenylation signal that separates the two expression cassettes thus preventing any transcription interference.

In transfected cells equipped with inflammasome components, ASC::GFP expression enables the visual monitoring of ASC specks, a hallmark of inflammasome activation .

The same plasmid is available with the GFP gene alone as a control (pSELECT-CGFP-zeo).

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Species
Human
Gene
ASC::GFP fusion gene
Plasmid backbone
pSELECT-zeo
Antibiotic resistance
Zeocin®
Gene promoter
hEF1-HTLV
Subclone start
AgeI
Subclone end
NheI
Purification
Ion-exchange chromatography
Reconstitution buffer
Sterile water
Quality control

Plasmid construct has been confirmed by restriction analysis and sequencing.

CONTENTS

Contents

  • Product: 
    pSELECT-hASC-GFP
  • Cat code: 
    psetz-hascgfp
  • Quantity: 
    20 µg
Includes:

1 ml of Zeocin™ (100 mg/ml)

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: Resuspended DNA should be stored at -20°C and is stable for 1 year.

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

pSELECT-hASC-GFP

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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