Anti-human Mincle (CLEC4E) antibody

Human Mincle neutralizing and detection antibody - Monoclonal Mouse IgG2b

Neutralizing and detection antibody against hMincle

Anti-hMincle-IgG (clone 15H5) is a monoclonal mouse IgG2b antibody specific for human (h) Mincle. This antibody was screened for neutralization activity and flow cytometry. It is produced in hybridomas and purified by affinity chromatography with protein G.

Macrophage-inducible C-type lectin (Mincle) is a C-type lectin receptor encoded by the C-type lectin domain family 4 member E (CLEC4E) gene. This receptor has emerged as an important player in innate immunity. It recognizes a variety of exogenous and endogenous stimuli, such as mycobacteria, some fungi, and necrotic cells [1, 2].

More details

Key features:

• Targets human Mincle
• Provided azide-free
• Each lot is functionally tested

Application

• Neutralization assay
• Flow cytometry

Read our review on C-Type Lectin Receptors

References:

1. Yamasaki S. et al., 2009. C-type lectin Mincle is an activating receptor for pathogenic fungus, Malassezia. PNAS 106(6): 1897–1902
2. Brown G.D., 2008. Sensing necrosis with Mincle. Nature Immunol. 9:1099-1100.

Specifications

Target: Human Mincle (hMincle)

Specificity: No cross-reactivity with murine Mincle

Clonality: Monoclonal antibody

Clone: 15H5

Isotype: Mouse IgG2b, kappa

Control: Mouse Control IgG2b

Source: Hybridoma cells

Formulation: 0.2 µm filtered solution in a sodium phosphate buffer with saccharose, glycine, and stabilizing agents.

Purity: Purified by affinity chromatography with protein G

Applications: Neutralization/block; Flow cytometry

Quality control:

• This product has been validated for neutralization using cellular assays.
• Binding of Anti-hMincle-IgG to hMincle on cells has been validated using flow cytometry.
• The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.

Contents

• 2 x 100 µg of purified Anti-hMincle-IgG, provided azide-free and lyophilized

Product is shipped at room temperature.

Upon receipt, store lyophilized antibody at -20°C.

Details

Mincle is a member of the C-type lectin receptor (CLR) family. Mincle recognizes a variety of exogenous and endogenous stimuli, such as mycobacteria, certain fungi, and necrotic cells [1, 2]. Exogenous ligands for Mincle include fungal α-mannose, and the mycobacterial glycolipid, trehalose-6’6’-dimycolate (TDM), also known as cord factor the immunostimulatory component of Mycobacterium tuberculosis [3].
Mincle also binds trehalose-6,6-dibehenate (TDB) which is a synthetic analog of TDM. Furthermore, Mincle senses damaged cells by recognizing endogenous damage-associated molecular patterns (DAMPs) [4]. Upon ligand recognition, Mincle interacts with the Fc receptor common γ-chain (FcRγ), which triggers intracellular signaling through Syk leading to CARD9-dependent NF-κB activation. Syk also induces the mobilization of intracellular calcium (Ca²⁺) and the activation of the calcineurin-NFAT pathway.

References:

1. Yamasaki S. et al., 2009​. C-type lectin Mincle is an activating receptor for pathogenic fungus, Malassezia. PNAS 106(6): 1897–1902. 
2. Brown GD. 2008.  Sensing necrosis with Mincle. Nature Immunol. 9:1099-1100. 
3. Ishikawa E. et al., 2009. Direct recognition of the mycobacterial glycolipid, trehalose dimycolate, by C-type lectin Mincle. J Exp Med. 206(13):2879-88.
4. Yamasaki S. et al., 2008. Mincle is an ITAM-coupled activating receptor that senses damaged cells. Nat Immunol. 9(10):1179-88.