Human Mincle (CLEC4E) Antibody

Mouse IgG2b (clone 15H5) - Hybridoma

ABOUT

Human Mincle neutralizing and detection antibody - Monoclonal Mouse IgG2b

Anti-hMincle-IgG (clone 15H5) is a monoclonal mouse IgG2b antibody specific for human (h) Mincle. This antibody was screened for neutralization activity and flow cytometry. It is produced in hybridomas and purified by affinity chromatography with protein G.

Macrophage-inducible C-type lectin (Mincle) is a C-type lectin receptor encoded by the C-type lectin domain family 4 member E (CLEC4E) gene. This receptor has emerged as an important player in innate immunity. It recognizes a variety of exogenous and endogenous stimuli, such as mycobacteria, some fungi, and necrotic cells [1, 2].

More details More details

 

Key features:

  • Targets human Mincle
  • Provided azide-free
  • Each lot is functionally tested

Application

  • Neutralization assay
  • Flow cytometry

 

Read our review Read our review on C-Type Lectin Receptors

 

References:

1. Yamasaki S. et al., 2009. C-type lectin Mincle is an activating receptor for pathogenic fungus, Malassezia. PNAS 106(6): 1897–1902
2. Brown G.D., 2008. Sensing necrosis with Mincle. Nature Immunol. 9:1099-1100.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Target

Mincle

Target species

Human

Species
Human
Isotype
mIgG2b
kappa
Clone
15H5
Tag
Tag-free
Source
Hybridoma
Purification
Protein G
Formulation buffer

Sodium phosphate buffer, glycine, saccharose, stabilizing agents

Preservative
Azide-free
Appearance (form)
Lyophilized
Reconstitution buffer
Sterile water (not provided)
Tested applications

Neutralization assay, flow cytometry

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    Anti-hMincle-IgG
  • Cat code: 
    mabg-hmcl-2
  • Quantity: 
    2 x 100 µg

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: -20°C for up to 1 year

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

Mincle is a member of the C-type lectin receptor (CLR) family. Mincle recognizes a variety of exogenous and endogenous stimuli, such as mycobacteria, certain fungi, and necrotic cells [1, 2]. Exogenous ligands for Mincle include fungal α-mannose, and the mycobacterial glycolipid, trehalose-6’6’-dimycolate (TDM), also known as cord factor the immunostimulatory component of Mycobacterium tuberculosis [3].
Mincle also binds trehalose-6,6-dibehenate (TDB) which is a synthetic analog of TDM. Furthermore, Mincle senses damaged cells by recognizing endogenous damage-associated molecular patterns (DAMPs) [4]. Upon ligand recognition, Mincle interacts with the Fc receptor common γ-chain (FcRγ), which triggers intracellular signaling through Syk leading to CARD9-dependent NF-κB activation. Syk also induces the mobilization of intracellular calcium (Ca2+) and the activation of the calcineurin-NFAT pathway.

 

References:

1. Yamasaki S. et al., 2009​. C-type lectin Mincle is an activating receptor for pathogenic fungus, Malassezia. PNAS 106(6): 1897–1902. 
2. Brown GD. 2008.  Sensing necrosis with Mincle. Nature Immunol. 9:1099-1100. 
3. Ishikawa E. et al., 2009. Direct recognition of the mycobacterial glycolipid, trehalose dimycolate, by C-type lectin Mincle. J Exp Med. 206(13):2879-88.
4. Yamasaki S. et al., 2008. Mincle is an ITAM-coupled activating receptor that senses damaged cells. Nat Immunol. 9(10):1179-88.

DOCUMENTS

Documents

Anti-hMincle-IgG

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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