Anti-human Dectin-1 (CLEC7A) antibody

Human Dectin-1 neutralizing and detection antibody - Monoclonal Mouse IgG1

Neutralizing and detection antibody against hDectin-1

Anti-hDectin-1-IgG (clone 22H8) is a monoclonal mouse IgG1 antibody against human Dectin-1 (hDectin-1). This antibody was screened for neutralization activity and flow cytometry. It is produced in hybridomas and purified by affinity chromatography with protein G.  Anti-hDectin-1-IgG recognizes hDectin-1 isoforms A and B. 

Dendritic cell-associated C-type lectin-1 (Dectin-1) is a C-type lectin receptor (CLR) that recognizes β-glucans, glucose polymers found in the cell walls of fungi, and thus plays an important role in antifungal innate immunity. Dectin-1  is encoded by the C-type lectin domain family 7 member A (CLEC7A) gene and alternatively spliced into 2 major isoforms: a full-length A isoform and a ‘stalkless’ B isoform, which do not induce the same response to soluble and particulate β-glucans [1, 2].

More details

Key features:

• Targets human Dectin-1
• Provided azide-free
• Each lot is functionally tested

Application

• Neutralization assay
• Flow cytometry

Read our review on Dectin-1 & β-glucans.

Read our review on Dectin-1 & inflammasomes.

References:

1. Brown G.D. et al., 2003. Dectin-1 mediates the biological effects of beta-glucans. J Exp Med. 197: 1119- 24.
2. Heinsbroek S.E. et al., 2006. C-type Lectin Receptor: Old Friend and New Player. Med Chem. 2017;13(6):536-543.

Specifications

Target: Human Dectin-1 (hDectin-1)

Specificity: No cross-reactivity with murine Dectin-1

Clonality: Monoclonal antibody

Clone: 22H8

Isotype: Mouse IgG1, kappa

Control: Mouse Control IgG1 

Source: Hybridoma cells

Formulation: 0.2 µm filtered solution in a sodium phosphate buffer with saccharose, glycine, and stabilizing agents.

Purity: Purified by affinity chromatography with protein G

Applications: Neutralization/block; Flow cytometry

Quality control:

• This product has been validated for neutralization using cellular assays.
• Binding of Anti-hDectin-1-IgG to hDectin-1 on cells has been validated using flow cytometry.
• The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.

Contents

• 2 x 100 µg of purified Anti-hDectin-1-IgG1 monoclonal antibody provided azide-free and lyophilized

Product is shipped at room temperature.

 Upon receipt, store lyophilized at -20°C.

Details

Dectin-1 is a member of the C-type lectin receptor (CLR) family and plays an important role in antifungal innate immunity. In humans and mice, it is alternatively spliced into two major isoforms, a full-length A isoform and a ‘stalkless’ B isoform [1, 2]. It is expressed on phagocytic cells, including macrophages and neutrophils.
Dectin-1 is a specific receptor for β-glucans [2]. β-Glucans are glucose polymers found in the cell wall of fungi, including the yeasts Saccharomyces cerevisiae and Candida albicans. Upon binding to its ligand, Dectin-1 triggers phagocytosis and signaling through the kinase Syk and the adaptors CARD9-Bcl10-Malt1 leading to the production of reactive oxygen species (ROS), activation of NF-κB and subsequent production of pro-inflammatory cytokines [3].
Notably, Dectin-1 signaling has been shown to collaborate with Toll‑like receptor 2 (TLR2) signaling to enhance the responses triggered by each receptor [4].

1. Fischer M. et al., 2017. Isoform localization of Dectin-1 regulates the signaling quality of anti-fungal immunity. Eur J Immunol. 47(5):848-859.
2. Hou H. et al., 2017. C-type Lectin Receptor: Old Friend and New Player. Med Chem. 13(6):536-543.
3. Drummond RA. & Lionakis MS., 2016. Mechanistic Insights into the Role of C-Type Lectin Receptor/ CARD9 Signaling in Human Antifungal Immunity. Front Cell Infect Microbiol. 6:39.
4. Romero MM. et al., 2016. Reactive oxygen species production by human dendritic cells involves TLR2 and dectin-1 and is essential for efficient immune response against Mycobacteria. Cell Microbiol. 18(6):875-86.