Poly(I:C) (LMW) Rhodamine

Rhodamine-labeled poly(I:C) (LMW)

Poly(I:C) Rhodamine was chemically labeled by covalent coupling of a rhodamine probe containing a reactive alkylating group. This confers fluorescent properties to poly(I:C) (LMW) whilst retaining TLR3 recognition properties.

Polyinosinic-polycytidylic acid (poly(I:C)) is a synthetic analog of double stranded RNA (dsRNA), a molecular pattern associated with viral infection.

Both natural and synthetic dsRNAs are known to induce type I interferons (IFN) and other cytokines production.

Poly(I:C) is recognized by Toll-like receptor 3 (TLR3).

InvivoGen provides poly(I:C) with a high molecular weight (HMW) or a low molecular weight (LMW) that might activate the immune system differently.

Specifications

Specificity: TLR3 agonist

Working Concentration: 100 ng - 10 μg/ml

Spectral Properties of Rhodamine

• Excitation λ max: 546 nm
• Emission λ max: 576 nm

Contents

• 10 µg Poly(I:C) Rhodamine
• 2 ml sterile endotoxin-free water

Poly(I:C) Rhodamine is shipped at room temperature

Stored at -20°C.

Protect from light.

Lyophilized product is stable 6 months at -20°C. Upon resuspension, Poly(I:C) Rhodamine should be aliquoted and stored at -20°C. 

Resuspended product is stable 3 months at -20°C.

Avoid repeated freeze-thaw cycles.