Anti-hACE2 mouse IgG2b

Anti-human ACE2 mouse monoclonal antibody

InvivoGen provides a mouse monoclonal antibody (mAb) featuring: 

• a variable region that specifically targets the human ACE2 transmembrane protein, established as the SARS-CoV-2 receptor [1]
• the mouse IgG2b constant region

The Anti-hACE2-mIgG2b antibody was obtained following Swiss mice immunization with DNA encoding hACE2. It is produced in hybridoma cells and purified by affinity chromatography with protein A.

Application

• Monitoring cell surface expression of human ACE2 in primary cells or engineered cell lines by FACS (see Figure)

Quality control

• Isotype confirmed by ELISA
• Functionality validated by FACS using HEK-Bue™ hACE2-TMPRSS2 cells

Read our reviews discussing SARS-CoV-2.

References

1. Hoffmann M. et al., 2020. SARS-CoV-2 cell entry depends on ACE2 and TMPRSS2 and is blocked by a clinically proven protease inhibitor. Cell. 181:1-16.