HKSP

Heat Killed Streptococcus pneumoniae

Streptococcus pneumoniae, a Gram positive bacterium, is the principal etiologic agent of bacterial meningitis in adults.

Heat-killed Streptococcus pneumoniae  (HKSP) induce activation of NF-κB in a TLR2- and CD14-dependent manner [1].

TLR2 has been shown to play an important role in the protein- and polysaccharide-specific type 1 IgG isotype response following immunization with HKSP [2].

References:

1. Yoshimura A. et al., 1999. Cutting Edge: Recognition of Gram-Positive Bacterial Cell Wall Components by the Innate Immune System Occurs Via Toll-Like Receptor 2. J. Immunol. 163:1–5.
2. Khan aQ. et al., 2005. Both Innate Immunity and Type 1 Humoral Immunity to Streptococcus pneumoniae Are Mediated by MyD88 but Differ in Their Relative Levels of Dependence on Toll-Like Receptor 2. Infect. Immun. 73: 298 - 307.

Specifications

Specificity: TLR2 agonist

Working concentration: 10⁷-10⁹ cells/ml

Solubility: Insoluble (resuspended HKSP results in a cloudy suspension)

Contents

HKSP is provided lyophilized.

• 10¹⁰ freeze-dried cells of Heat Killed Streptococcus pneumoniae (HKSP)
• 1.5 ml sterile endotoxin-free water

HKSP is shipped at room temperature.

Store at 4°C.

Upon resuspension, prepare aliquots of HKSP and store at 4°C for short term storage or -20°C for long storage.

Product is stable 1 month at 4°C and 6 months at -20°C when properly stored.