TLR::GFP Fusions

TLR::GFP fusion genes were generated by fusing the green fluorescent protein (GFP) gene to the C terminus of various human Toll-like receptor (TLR) genes (TLR1 to TLR6).

These TLR::GFP fusion proteins can be used to study the localization of the TLRs. Transfected cells can be analyzed for GFP expression by flow cytometry and Western-blotting using GFP antibodies.

The TLR-GFP fusion genes are cloned in the pUNO1 plasmid under the control of the strong and ubiquitous mammalian promoter EF1α/HTLV.

The pUNO1 plasmid is selectable with blasticidin in both E. coli and mammalian cells.

Products

TLR1::GFP		Plasmid expressing the human TLR1 gene fused to a GFP gene
TLR2::GFP		Plasmid expressing the human TLR2 gene fused to a GFP gene
TLR3::GFP		Plasmid expressing the human TLR3 gene fused to a GFP gene
TLR4::GFP		Plasmid expressing the human TLR4 gene fused to a GFP gene
TLR5::GFP		Plasmid expressing the human TLR5 gene fused to a GFP gene
TLR6::GFP		Plasmid expressing the human TLR6 gene fused to a GFP gene