Spike-RBD-His
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Cat.code:
his-sars2-rbd
- Documents
ABOUT
SARS-CoV-2 Spike RBD with C-terminal His tag
Protein description
The SARS-CoV-2 (2019-nCoV) Spike RBD (receptor binding domain) has been identified as the key viral element allowing the virus docking to the target cells. RDB is recognized by the ACE2 surface membrane receptor [1-3]. RBD is a candidate for subunit prophylactic vaccines against SARS-CoVs [4, 5]. RBD is also at the center of therapeutic approaches, such as the development and testing of small peptide inhibitors or soluble ACE2 to block the SARS-CoV-2 entry into target cells [6].
Spike-RBD-His was generated by fusing the C-terminus of SARS-CoV-2 Spike RBD [R319-F541] to a poly-histidine sequence (6 x His).
The SARS-CoV-2 viral sequence used is from the Wuhan-Hu-1 (D614) isolate.
This protein has been produced in CHO cells and purified by affinity chromatography (See Details and Specifications for more information).
Applications
- Vaccination studies: using combinations of Spike protein antigens and adjuvants
- Antibody screening: finding anti-Spike antibodies that can neutralize the SARS-CoV-2 infection
- Inhibitor screening: finding small molecules, or antibodies able to block the SARS-CoV-2 RBD interaction with the ACE2 receptor
- ACE2 cellular expression screening: in primary isolated cells or transfected cells
Quality control
- Size and purity confirmed by SDS PAGE
- Protein validated by ELISA using a coated Anti-SARS-CoV-Spike-RBD hIgM mAb (clone CR3022)
Learn more about SARS-CoV-2 infection cycle, immune responses, and potential therapeutics.
References
1. Li F., 2016. Structure, function, and evolution of coronavirus spike proteins. Annu. Rev. Virol. 3:237-261.
2. Li F. et al., 2005. Structure of SARS coronavirus spike receptor-binding domain complexed with receptor. Science. 309:1864-1868.
3. Walls A.C. et al., 2020. Structure, function, and antigenicity of the SARS-CoV-2 spike glycoprotein. Cell. 181(2):281-292.e6.
4. Wang N. et al., 2020. Subunit vaccines against emerging pathogenic human coronaviruses. Front. Microbiol. 11:298. DOI: 10.3389/fmicb.2020.00298.
5. Padron-Regalado E., 2020. Vaccines for SARS-CoV-2: Lessons from other coronavirus strains. Infect. Dis. Ther. DOI: 10.1007/s40121-020-00300-x.
6. Monteil V.et al., 2020. Inhibition of SARS-CoV-2 infections in engineered human tissues using clinical-grade soluble human ACE2. Cell. 181:1-9.
All InvivoGen products are for internal research use only, and not for human or veterinary use.
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SPECIFICATIONS
Specifications
YP_009724390
Sodium phosphate buffer, glycine, saccharose, and stabilizing agents
Negative (tested using EndotoxDetect™ assay)
Cellular assays, ELISA
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:Spike-RBD-His
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Cat code:his-sars2-rbd
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Quantity:50 µg
1.5 ml of endotoxin-free water
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
DOCUMENTS
Documents
Technical Data Sheet
Validation Data Sheet
Plasmid Sequence
Safety Data Sheet
Certificate of analysis
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