ODN INH-18 - TLR9 Antagonist

TLR9 antagonist - Class B inhibitory ODN oligonucleotide

Toll-Like Receptor 9 (TLR9) detects unmethylated CpG dinucleotides in bacterial or viral DNA inducing strong immunostimulatory effects.

TLR9 activation can be mimicked by synthetic phosphorothioate-stabilized oligodeoxynucleotides (ODN) containing immune stimulatory “CpG motifs”.

Studies suggest the existence of DNA sequences that can neutralize the stimulatory effect of CpG ODNs.

ODN INH-18 is a linear and class R (‘restricted’) inhibitory ODN. It contains an inhibitory DNA motif consisting of two nucleotide triplets, a proximal CCT and a more distal GGG, spaced from each other by four nucleotides.

ODN INH-18 is a potent inhibitor of TLR9-induced B cells and macrophages [1]. ODN INH-18 strongly blocks TLR9 activation in both human and mouse TLR9-expressing cells.

Reference:

1. Lenert P. et al., 2009. DNA-like class R inhibitory oligonucleotides (INH-ODNs) preferentially block autoantigen-induced B-cell and dendritic cell activation in vitro and autoantibody production in lupus-prone MRL-Fas(lpr/lpr) mice in vivo. Arthritis Res Ther. 11(3):R79.