Anti-hIL-6 Neutralizing mAb
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Anti-hIL-6-IgG Human IL-6 Neutralizing Antibody (clone 3H3) - Monoclonal Mouse IgG1 |
Show product |
3 x 100 µg |
mabg-hil6-3
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Neutralizing monoclonal antibody against human interleukin 6
Neutralizing monoclonal antibody against human IL-6
Anti-hIL-6-IgG (clone 3H3) is a monoclonal antibody (mAb) specific for human interleukin 6 (hIL-6). It is produced in hybridomas and purified by affinity chromatography. This antibody has been selected for its ability to efficiently neutralize the biological activity of hIL-6. IL-6 is a multifunctional cytokine that plays an important role in the regulation of the immune response, hematopoiesis, and inflammation [1, 2].
Key features:
- A potent and specific neutralizing mAb against hIL-6
- Provided azide-free
- No cross-reactivity with murine IL-6
- Each lot is functionally tested
References:
1. Kang S. et al., 2015. Therapeutic uses of anti-interleukin-6 receptor antibody. Int. Immunol. 27: 21-9.
2. Akira S. et al., 1990. Biology of multifunctional cytokines: IL 6 and related molecules (IL 1 and TNF). FASEB J. 4(11):2860-7.
Figures
Specifications
Target: Natural and recombinant human interleukin 6 (IL-6)
Specificity: No cross-reactivity with mouse IL-6
Clone: 3H3
Isotype: Mouse IgG1, kappa
Control: Mouse IgG1 Control
Immunogen: Human IL-6 protein expressed in Swiss mice following DNA immunization
Formulation: 0.2 µm filtered solution in a sodium phosphate buffer with glycine, saccharose, and stabilizing agents
Tested applications: Blocking & Neutralisation
Quality control:
- This product has been validated for neutralization.
- The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.
Contents
- 3 x 100 μg purified anti-hIL-6-IgG antibody, provided azide-free and lyophilized.
The product is shipped at room temperature.
Upon receipt, store lyophilized antibody at -20 °C.
Reconstituted antibody is stable for 1 month at 4 °C and for 1 year at -20 °C.
Avoid repeated freeze-thaw cycles.
Details
The proinflammatory cytokine interleukin 6 (IL-6) is one of the most important mediators of fever and of the acute phase response [1, 2]. IL-6 exerts its action by first binding to the IL-6 receptor (IL-6R). The complex of IL-6 and IL-6R associates with the signal-transducing membrane protein gp130, thereby inducing its dimerization.
This leads to the activation by phosphorylation of the tyrosine kinases of the Janus family (JAK1, JAK2, and Tyk2). Activated JAKs induce dimerization and translocation to the nucleus of STAT3 where it binds enhancer elements of IL-6-inducible genes [3].
In HEK-Blue™ IL-6 cells activation of the JAK/STAT3 pathway with IL-6 leads to the production of the secreted alkaline phosphatase (SEAP) reporter.
1. Kang S. et al., 2015. Therapeutic uses of anti-interleukin-6 receptor antibody. Int. Immunol. 27: 21-9.
2. Akira S. et al., 1990. Biology of multifunctional cytokines: IL 6 and related molecules (IL 1 and TNF). FASEB J. 4(11):2860-7.
3. Murray P., 2007. The JAK-STAT Signaling Pathway: Input and Output Integration. J. Immunol. 178:2623-9.