Anti-hIL-6-IgG
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Cat.code:
mabg-hil6-3
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ABOUT
Neutralizing monoclonal antibody against human interleukin 6
Anti-hIL-6-IgG (clone 3H3) is a monoclonal antibody (mAb) specific for human interleukin 6 (hIL-6). It is produced in hybridomas and purified by affinity chromatography. This antibody has been selected for its ability to efficiently neutralize the biological activity of hIL-6. IL-6 is a multifunctional cytokine that plays an important role in the regulation of the immune response, hematopoiesis, and inflammation [1, 2].
Key features:
- A potent and specific neutralizing mAb against hIL-6
- Provided azide-free
- No cross-reactivity with murine IL-6
- Each lot is functionally tested
References:
1. Kang S. et al., 2015. Therapeutic uses of anti-interleukin-6 receptor antibody. Int. Immunol. 27: 21-9.
2. Akira S. et al., 1990. Biology of multifunctional cytokines: IL 6 and related molecules (IL 1 and TNF). FASEB J. 4(11):2860-7.
All products are for research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
IL-6
Human
Neutralization assay
Sodium phosphate buffer, glycine, saccharose, stabilizing agents
Neutralization assay
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:Anti-hIL-6-IgG
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Cat code:mabg-hil6-3
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Quantity:3 x 100 µg
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
Details
The proinflammatory cytokine interleukin 6 (IL-6) is one of the most important mediators of fever and of the acute phase response [1, 2]. IL-6 exerts its action by first binding to the IL-6 receptor (IL-6R). The complex of IL-6 and IL-6R associates with the signal-transducing membrane protein gp130, thereby inducing its dimerization.
This leads to the activation by phosphorylation of the tyrosine kinases of the Janus family (JAK1, JAK2, and Tyk2). Activated JAKs induce dimerization and translocation to the nucleus of STAT3 where it binds enhancer elements of IL-6-inducible genes [3].
In HEK-Blue™ IL-6 cells activation of the JAK/STAT3 pathway with IL-6 leads to the production of the secreted alkaline phosphatase (SEAP) reporter.
1. Kang S. et al., 2015. Therapeutic uses of anti-interleukin-6 receptor antibody. Int. Immunol. 27: 21-9.
2. Akira S. et al., 1990. Biology of multifunctional cytokines: IL 6 and related molecules (IL 1 and TNF). FASEB J. 4(11):2860-7.
3. Murray P., 2007. The JAK-STAT Signaling Pathway: Input and Output Integration. J. Immunol. 178:2623-9.
DOCUMENTS
Documents
Technical Data Sheet
Validation Data Sheet
Safety Data Sheet
Certificate of analysis
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